411550

Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate

liquid, clone HP6043, Calbiochem^®

• NACRES: NA.46
• UNSPSC Code: 12352203
• Clone: HP6043, monoclonal
• Species reactivity: -
• Application: —
• Citations: 4

Właściwości

• biological source: mouse
• Quality Segment: 200
• antibody form: purified antibody
• antibody product type: secondary antibodies
• clone: HP6043, monoclonal
• form: liquid
• does not contain: preservative
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −20°C

Opis

General description

Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes human IgG Fc fragment.

This Mouse Anti-Human IgG, Fc Fragment Specific (HP6043) Peroxidase Conjugate is validated for use in Enzyme Immunoassay for the detection of Human IgG, Fc Fragment Specific.

Application

Enzyme Immunoassay (1:1000, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 10 mg/ml BSA.

Other Notes

Antibody should be titrated for optimal results in individual systems.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.2%

Human IgA: <0.1%

Human IgE: <0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG₁: 100%

Human IgG₂: 100%

Human IgG₃: 100%

Human IgG₄: 100%

Human IgM: <0.01%

Human IgA: <0.01%

Human IgE: <0.01%



There is no detectable binding to bovine, goat, horse sheep IgG, so these species sera can be used for assay blocking buffers. Modification of Nakane and Kawaoi method used for enzyme coupling.

Hamilton, R.G. 1993. Clin. Chem.39, 1988.
Hamilton, R.G. and Morrison, S.M. 1993. J. Immunolo. Meth.158, 107.
Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Hamilton, R.G., et al. 1988. J. Immunoassay.9, 275.
Hamilton, R.G., et al. 1987. Hybridoma6, 204.
Nakane, P.K. and Kawaoi A., 1974. J. Histochem Cytochem.22, 1084.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable