As per our CofA for # 20-188:
For use in mammalian cell lysis. Dilute one part 10XRIPA Lysis Buffer with nine parts sterile, distilled
water. Stir mixture and refrigerate at least two hours prior to use. Immediately prior to use add the
protease and phosphatase inhibitors for the following final concentrations: 1mM PMSF, 1mM sodium orthovanadate, 1mM sodium fluoride, 1µg/ml aprotinin, 1µg/ml leupeptin, 1µg/ml pepstatin.
Storage and Stability: Stable for 6 months at room temperature from date of shipment.
Store in the dark.
20-188
Bufor do lizy RIPA, 10X
100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.
Synonim(y):
bufor lizujący
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 533,00 zł |
Informacje o tej pozycji
UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
533,00 zł
Dostępne do wysyłki DZISIAJSzczegóły
Pomoc techniczna
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Pozwól nam pomócform
liquid
packaging
pkg of 100 mL
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
shipped in
ambient
General description
Radioimmunoprecipitation assay (RIPA) Buffer aids in cell lysis and solubilization of proteins from both adherent and suspension-cultured mammalian cells. In addition, this buffer helps in relevant protein-protein interactions studies by reducing non-specific protein-binding interactions to keep background low, while allowing most specific interactions to occur.
Application
Physical form
0.5M Tris-HCl, pH 7.4, 1.5M NaCl, 2.5% deoxycholic acid, 10% NP-40, 10mM EDTA.
Preparation Note
6 months at room temperature
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja | |||
|---|---|---|---|
| technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) immunoprecipitation (IP): suitable | technique(s) - | technique(s) Southern blotting: suitable |
| form liquid | form liquid | form solution | form solution |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| packaging pkg of 100 mL | packaging pkg of 50 mL, pkg of 500 mL | packaging pkg of 100 mL | packaging - |
| manufacturer/tradename Upstate® | manufacturer/tradename - | manufacturer/tradename Roche | manufacturer/tradename - |
signalword
Danger
Klasa składowania
10 - Combustible liquids
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Dam. 1
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CAS No.
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CAS No.
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CAS No.
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| 20-188 | 04053252677526 |
| BAH369400010-10EA | 04065269242932 |
| BAH369401010-10EA | 04065269242949 |
| BAH369400000-200EA | 04065269242925 |
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where is the recommended protocol for this product?
1 answer-
Helpful?
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Can I prepare 10 ml of 1x RIPA buffer and add phosphatase and protease inhibitor then aliquot it to 1 ml Eppendorf tubes and store at -20 for use?
1 answer-
Freeze-thaw cycles may lead to degradation of added phosphatase and protease inhibitors. It is highly recommended to prepare the cocktail just prior to application.
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Can this lysis extract protein from the Nucleus?
1 answer-
RIPA Buffer enables the extraction of cytoplasmic, membrane, and nuclear proteins. Certain proteins are more soluble in RIPA buffer than others.
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How long can 1x RIPA lysis buffer be stored with or without the protease?
1 answer-
This product is stable at room temperature for 6 months from date of shipment. Protease and phosphatase inhibitors should be added immediately prior to use.
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I used the 10X RIPA buffer to lyse my cultured cells. How can I do next? Will it affect the protein quantification by using BCA assay kit? Also, will it affect the SDS-PAGE and Western blot?
1 answer-
Protein samples prepared from cell culture lysates using this 1X RIPA buffer are typically compatible with downstream applications such as BCA assay, SDS-PAGE, and Western blotting. The concentrations of salts and detergents present in the 1X RIPA buffer are relatively low, minimizing interference with these techniques.
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Can we store it a 4 degree, if we want to use it after 6 months?
1 answer-
Studies have not been performed to determine if refrigerated storage will extend the shelf life of this product. The end user would have to determine suitability if used after the recommended 6 months. Note that product R0278, a 1X Ripa Buffer, is stored refrigerated and includes a warranty of 1 year from the date of shipment. Please see the link below to review this product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdfHelpful?
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Someone in lab left the 10X RIPA Buffer in room temperature for overnight. Will it affect the quality of the buffer? Could we continue to use it?
1 answer-
This product does not require refrigeration and is stable at room temperature for up to 6 months.
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Do I used distilled water to dilute to 1X?
1 answer-
The 10X RIPA Lysis Buffer should be diluted with nine parts sterile, distilled water.
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Do I have to dilute to 1X for protein isolation?
1 answer-
Yes, RIPA lysis buffer should be diluted to 1X prior to use.
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