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17-10461

EZ-Magna ChIP® Zestaw do immunoprecypitacji chromatyny HiSens

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Gabaryty przesyłkiSKUDostępnośćCena netto
24 assays
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2700,00 zł

Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
41105331

2700,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Quality Segment

100

General description

The EZ-Magna HiSens ChIP kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results.

Application

Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to enable ChIP from low input amounts of chromatin obtained from either cells or tissues using magnetic A/G beads. Control primers included.

Packaging

Kit capacity: 24 chromatin immunoprecipitation assays

Other Notes

10X Glycine;Nuclei Isolation Buffer;10X PBS;SCW Buffer (Sonication/ChIP/Wash);Magna ChIP Protein A/G Magnetic Beads;Low Stringency IP Wash Buffer;ChIP Elution Buffer;Protease Inhibitor Cocktail;Proteinase K Solution;Anti-Trimethyl-Histone H3 (Lys4);Normal Rabbit IgG;Control PCR primers

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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17-40817-1046017-10078
EZ-Magna ChIP® HiSens Chromatin Immunoprecipitation Kit

Sigma-Aldrich

17-10461

EZ-Magna ChIP® HiSens Chromatin Immunoprecipitation Kit

EZ-Magna ChIP® A - Chromatin Immunoprecipitation Kit Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads. Control primers included.

Sigma-Aldrich

17-408

EZ-Magna ChIP® A - Chromatin Immunoprecipitation Kit

Sigma-Aldrich

Sigma-Aldrich

17-10460

Magna ChIP® HiSens Chromatin Immunoprecipitation Kit

EZ-Magna ChIP® HT96 Chromatin Immunoprecipitation Kit

Sigma-Aldrich

17-10078

EZ-Magna ChIP® HT96 Chromatin Immunoprecipitation Kit

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

pictograms

CorrosionEnvironment
GHS05,GHS09

signalword

Danger

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

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Powiązane treści

Magna ChIP™ HiSens Kits

Superior enrichment, low background. With performance proven for both qPCR and ChIP-seq analysis, the Magna ChIP™ HiSens kit may be the only ChIP kit you’ll ever need. Outperforming any competing kit, this revolutionary approach to ChIP enables enrichment from both low and high amounts of input chromatin while also delivering low backgrounds and high signal-to-noise ratios for ultra-sensitive detection.

Magna ChIP™ HiSens Kits

Superior enrichment, low background. With performance proven for both qPCR and ChIP-seq analysis, the Magna ChIP™ HiSens kit may be the only ChIP kit you’ll ever need. Outperforming any competing kit, this revolutionary approach to ChIP enables enrichment from both low and high amounts of input chromatin while also delivering low backgrounds and high signal-to-noise ratios for ultra-sensitive detection.

Getting started with ChIP-seq: experimental design to data analysis

Chromatin-immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-seq) of the immunoprecipitated DNA is a powerful tool for the investigation of protein:DNA interactions. To perform ChIP-seq, chromatin is isolated from cells or tissues (with or without chemical crosslinking) and fragmented. Antibodies recognizing chromatinassociated proteins of interest are used to enrich the sample for specific chromatin fragments. The DNA is recovered, sequenced on various NGS platforms, and aligned to a reference genome to determine specific protein binding loci. ChIP-seq studies have increased our knowledge of transcription factor biology, DNA methylation and histone modifications.

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