14-863
Raf-1 RBD Protein, GST, 300 µg
GST fusion-protein, corresponding to the human Ras Binding Domain (RBD, residues 1-149) of Raf-1, expressed in E. coli. with purity 50% at full length molecular weight 42 kDa. For use in Affinity Binding Assays
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About This Item
Kod UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
human
Poziom jakości
Postać
liquid
okres trwałości
1 yr
masa cząsteczkowa
Mw 42 kDa
producent / nazwa handlowa
Upstate®
stężenie
>50%
metody
affinity binding assay: suitable
rozpuszczalność
water: soluble
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
informacje o genach
human ... RAF1(5894)
Opis ogólny
Research Area: Cell signaling
The rapidly accelerated fibrosarcoma (Raf) family, consisting of three highly conserved cytosolic serine/threonine kinases (ARAF, BRAF, and RAF1 or CRAF) is characterized by three conserved regions such as CR1, CR2, and CR34. The RAS-binding domain (RBD) and the cysteine-rich domain (CRD) are found in the N-terminal CR1 region.
The rapidly accelerated fibrosarcoma (Raf) family, consisting of three highly conserved cytosolic serine/threonine kinases (ARAF, BRAF, and RAF1 or CRAF) is characterized by three conserved regions such as CR1, CR2, and CR34. The RAS-binding domain (RBD) and the cysteine-rich domain (CRD) are found in the N-terminal CR1 region.
Zastosowanie
Research Category
Metabolism
Metabolism
Research Sub Category
Obesity
Metabolic Disorders
Obesity
Metabolic Disorders
Specifically binds to and precipitates Ras-GTP from cell lysate.Can be bound to glutathione coated plates prior to precipitating Ras-GTP.
Działania biochem./fizjol.
c-Raf-1 acts as an effector of Ras and is a serine-threonine kinase that transmits incoming signals to the mitogen-activated protein kinase pathway. This pathway is associated with various cellular responses, including cell proliferation, survival, and differentiation. RBD directly binds to the Ras·GTP complex in vitro. Furthermore, bacterially expressed recombinant RBD assumes a stable folded conformation in vitro. The GST·RBD fusion protein, linked to Sepharose beads may be used to precipitate oncogenic Leu61-Ha-Ras, which is constitutively bound with GTP.
Jakość
Affinity Precipitation of Activated Ras: Raf-1-RBD, GST can be captured on a glutathione coated plate and used to capture GTP activated Ras from HeLa cell lysate. Used 2 μg of Raf-1-RBD, GST per well to capture 25-100 μg of EGF stimulated HeLa cell lysate. Upstate also offers a Ras GTPase ELISA kit (17-424).
Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.
Note: Due to spontaneous deactivation of Ras, use lysates that have been stored at -70°C for less than 6 month.
Postać fizyczna
300 μg of Raf-1 RBD, GST in 150 μL in PBS, pH 7.4, 0.5mM PMSF, and 20% glycerol.
Glutathione-agarose
Przechowywanie i stabilność
1 year at -20°C from date of shipment.
Informacje prawne
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Certyfikaty analizy (CoA)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
R Wynn et al.
Protein science : a publication of the Protein Society, 6(8), 1621-1626 (1997-08-01)
We have determined by X-ray crystallography the structures of several variants of staphylococcal nuclease with long flexible straight chain and equivalent length cyclic unnatural amino acid side chains embedded in the protein core. The terminal atoms in the straight side
Point mutants of c-raf-1 RBD with elevated binding to v-Ha-Ras
Fridman M, et al.
The Journal of biological chemistry, 275(39), 30363-30371 (2000)
Timothy H Tran et al.
Nature communications, 12(1), 1176-1176 (2021-02-21)
The first step of RAF activation involves binding to active RAS, resulting in the recruitment of RAF to the plasma membrane. To understand the molecular details of RAS-RAF interaction, we present crystal structures of wild-type and oncogenic mutants of KRAS
M Foschi et al.
The EMBO journal, 16(21), 6439-6451 (1997-11-14)
Endothelin-1 (ET-1) induces cell proliferation and differentiation through multiple G-protein-linked signaling systems, including p21ras activation. Whereas p21ras activation and desensitization by receptor tyrosine kinases have been extensively investigated, the kinetics of p21ras activation induced by engagement of G-protein-coupled receptors remains
J de Rooij et al.
Oncogene, 14(5), 623-625 (1997-02-06)
Ras is a small GTPase that cycles between an inactive GDP-bound and an active GTP-bound form. A large variety of ligands that stimulate cell surface receptors induce the activation of Ras. Thus far, this activation could only be measured by
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