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Merck

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12-342

Królicze przeciwciało IgG przeciw owcom, koniugat HRP

Upstate®, from rabbit

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Gabaryty przesyłkiSKUDostępnośćCena netto
500 μg
Sprawdź dostępność w koszyku
988,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA, IHC, WB
Citations:
29

988,00 zł


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biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

sheep

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate

Preparation Note

Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.

Analysis Note

Routinely evaluated by western blot

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Ta pozycja
AP147P12-34812-349
antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

technique(s)

ELISA: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

biological source

rabbit

biological source

rabbit

biological source

goat

biological source

goat

species reactivity

sheep

species reactivity

sheep

species reactivity

rabbit

species reactivity

mouse

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal


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Klasa składowania

11 - Combustible Solids

wgk

WGK 3



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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów



Susannah L McKay et al.
PloS one, 6(1), e16077-e16077 (2011-02-02)
There is mounting evidence to suggest that the synthesis of pre-mRNA transcripts and their subsequent splicing are coordinated events. Previous studies have implicated the mammalian spliceosomal U2 snRNP as having a novel role in stimulating transcriptional elongation in vitro through
Donel A Sequea et al.
Mechanisms of ageing and development, 134(1-2), 60-63 (2012-12-19)
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence
Naveen Sharma et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(12), R1261-R1267 (2012-11-02)
Akt is a serine/threonine kinase that plays a key role in numerous cellular functions including metabolism, growth, protein synthesis, apoptosis, and cell proliferation. The most consistent and robust effect of moderate calorie restriction (CR; ~60% of ad libitum, AL, food



Questions

1–2 of 2 Questions  
  1. Could you please provide the reconstitution method for this product and the recommended concentration for various uses?

    1 answer
    1. To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.

      Helpful?

  2. What is the method of reconstitution for this product and what is the concentration recommended for various uses?

    1 answer
    1. This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation

      Helpful?

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