12-348

Kozie przeciwciało IgG przeciw królikowi, koniugat HRP

1 mg/mL, Upstate^®

• Synonim(y): IgG przeciw królikowi
• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: ELISA, IHC, WB
• Citations: 433

Właściwości

• biological source: goat
• Quality Segment: 100
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: rabbit
• manufacturer/tradename: Upstate^®
• concentration: 1 mg/mL
• technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• shipped in: dry ice
• target post-translational modification: unmodified

Opis

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Immunogen

Highly purified whole rabbit IgG.

Application

Goat Anti-Rabbit IgG Antibody, HRP-conjugate detects level of Rabbit IgG & has been published & validated for use in WB, IH, ELISA.

Biochem/physiol Actions

Specific for rabbit IgG heavy and light chains.

Physical form

Goat polyclonal conjugated with horseradish peroxidase IgG in buffer containing 50% storage buffer 0.02 M potassium phosphate, 0.15 M NaCl, pH 7.2, 10 mg/mL BSA, 0.01% (w/v) gentamicin sulfate and 50% glycerol.
Rehydration: Add 500 µL sterile, distilled water containing 50% glycerol, to make a 1 mg/mL stock solution.

Analysis Note

Evaluated by western blot.

Western Blot Analysis: Suitable for western blotting (dot blot), ELISA and immunohistochemistry. A working dilution of 1:15,000 to 1:60,000 is suggested for ELISA. A working dilution of 1:2,000 to 1:6,000 is suggested for western blotting procedures. A working dilution of 1:500 to 1:3,000 is suggested for immunohistochemistry. Optimal antibody dilution for other applications should be determined by the researcher.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3