T8690
Thioredoxin human
≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg
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About This Item
Polecane produkty
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in E. coli (N-terminal histidine tagged)
Próba
≥90% (SDS-PAGE)
Postać
essentially salt-free, lyophilized powder
aktywność właściwa
≥5 U/mg
masa cząsteczkowa
14 kDa
metody
activity assay: suitable
rozpuszczalność
water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
przydatność
suitable for molecular biology
numer dostępu UniProt
Zastosowanie
cell analysis
temp. przechowywania
−20°C
informacje o genach
human ... TXN(7295)
Opis ogólny
Cloned from Jurkat cell cDNA. Identical to the ADF/Trx (GenBank® Accession Number X77584) and the thioredoxin from human placenta (GenBank® Accession Number AF085844).
Thioredoxin (TXN) is a 12 kDa ubiquitous protein. The gene is located on human chromosome 9q31.3. TXN is mainly located in the cytoplasm.Thioredoxin contains a redox-active dithiol in the active site. Itexists in several forms, the most prevalent ones being the cytosolic (TRX1) andmitochondrial (TRX2) forms.
Zastosowanie
Thioredoxin human can be used for in vitro thioredoxin assay. The product can also be used for assessing Thioredoxin reductase (TRXR) enzyme activity.
Thioredoxin human has been used:
- in mitogen-activated protein kinase kinase 4 (MKK4) in vitro kinase assay
- to study its role in transcription regulation, RNA processing and nuclear pore function in human cells
- in proteomics identification of Trx1 reduction target proteins from the hearts of cardiac specific transgenic (Tg)-Trx1 mice
Działania biochem./fizjol.
Human Thioredoxin is a recombinant, N-terminal histidine tagged protein. The product induces proliferation in lymphoid cells, fibroblasts as well as in numerous human solid tumor cell lines.The product also stimulates growth in normal as well as leukemic B cell, modifies the transcriptional activity of AP-1and inhibits the apoptosis signal-regulating kinase 1 (ASK1).Thioredoxin (TRX) plays a role in protecting the cells fromelectrophiles, a cytotoxic agent.
Thioredoxin (TXN) has disulfide-reducing activity. It acts as a cytokine to reduce inflammation. TXN functions as a scavenger for reactive oxygen intermediates (ROIs), which are produced in various oxidative stress. Thioredoxin induces cellular proliferation to decrease oxidative stress. TXN is a prognostic biomarker for various cancers, such as lung, pancreatic and hepatocellular carcinoma. It contributes to protein reduction, tissue development and redox regulation.
Mediates the reduction of disulfide bonds in proteins.
Definicja jednostki
One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.
Informacje prawne
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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