SRP6045

Glucokinase from human liver

recombinant, expressed in E. coli, ≥77% (SDS-PAGE)

• Synonim(y): Hexokinase, Hexokinase, liver, Liver glucokinase
• Numer CAS: 9001-51-8
• Kod UNSPSC: 12352204
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human liver
• rekombinowane: expressed in E. coli
• Próba: ≥77% (SDS-PAGE)
• Postać: liquid
• masa cząsteczkowa: 53.4 kDa
• opakowanie: pkg of 10 μg
• stężenie: ~1 mg/mL
• zanieczyszczenia: Endotoxin, tested
• numer dostępu NCBI: P35557
• numer dostępu UniProt: P35557
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... GCK(2645)

Opis

Opis ogólny

The gene encoding glucokinase (GCK) is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The enzyme is expressed as a monomer and possesses one glucose-binding site. The gene encoding GCK is localized on human chromosome 7. Recombinant human liver glucokinase (Accession# NP_277042)) has a C-terminal FLAG tag and has 474 amino acid residues.

Działania biochem./fizjol.

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. The main function of glucokinase (GCK) is to regulate carbohydrate metabolism. The enzyme triggers the release of insulin in β-cells of pancreas. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity. Mutation in the gene encoding this protein is associated with a rare form of diabetes and the protein may also have a role in type 2 diabetes.

Definicja jednostki

One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30°C. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2 mM ATP, 25 mM glucose, 100 μg/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human liver glucokinase at 30°C for 30 min.

Postać fizyczna

50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable