Safranin O stain for cartilage requires a specific procedure involving fixative, deparaffinization, staining, and mounting. The use of acetic acid after staining sets the stain to the tissue.
Przejdź do
S8884
Safranin O
Dye content, ≥80%, certified by the Biological Stain Commission, powder
Synonim(y):
Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 25 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 658,00 zł | |
| 100 g | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2250,00 zł |
Informacje o tej pozycji
Wzór empiryczny (zapis Hilla):
C20H19ClN4
Numer CAS:
Masa cząsteczkowa:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
207-518-8
MDL number:
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
658,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócNazwa produktu
Safranin O, certified by the BSC
agency
certified by the BSC
Quality Segment
form
powder
composition
Dye content, ≥80%
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
H2O: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N
InChI
1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H
InChI key
OARRHUQTFTUEOS-UHFFFAOYSA-N
General description
Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.
Application
Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.
Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.
Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) titration: suitable | technique(s) cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable |
| color green to very dark green | color - | color - | color - |
| form powder | form powder | form powder or crystals | form - |
| solubility H2O: 1 mg/mL | solubility water: 1 mg/mL, clear, dark red to very dark red and red purple | solubility methanol: 0.01 g/10 mL, red | solubility - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
Still not finding the right product?
Explore all of our products under Safranin O
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Gloves
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Powiązane treści
Isolation of Multipotent Mesenchymal Stem Cells from Human Extraocular Muscle Tissue
Sharma A, et al.
Bio-protocol, 9(4) (2016)
Paul A. Banaszkiewicz
Classic Papers in Orthopaedics (2014)
Novel markers of osteogenic and adipogenic differentiation of human bone marrow stromal cells identified using a quantitative proteomics approach
CeciliaGraneli
Stem Cell Research (2014)
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| S8884-100G | 04061835574049 |
| S8884-25G | 04061836961138 |
-
Is there a staining protocol available for staining cartilage, particularly for decalcified bone (joint tissue) using CalX, and subsequent staining with Fast green and Safranin O?
1 answer-
Helpful?
-