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R8257
Mlu I from Micrococcus luteus (lysodeikticus)
buffered aqueous glycerol solution
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
Formularz
buffered aqueous glycerol solution
stężenie
10,000 units/mL
Warunki transportu
wet ice
temp. przechowywania
−20°C
Specyficzność
Recognition sequence: 5′-A/CGCGT-3′
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Ligation and recutting results: After 2-10-fold Mlu I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: Partially inactivated at 65 °C for 10 minutes.
Inne uwagi
Supplied with 10x Restriction Endonuclease Buffer SH (B3657).
Przestroga
Comment: Mlu I will only partially cleave DNA isolated from E. coli strains that have the dam methylase (dam+ strains).
Postać fizyczna
Solution in 20 mM Tris-HCl, pH 8.0, 0.1 mM EDTA, 250 mM KCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.2% Triton X-100 (v/v) at 4°C
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Two new restriction endonucleases have been isolated from Flavobacterium okeanokoites IFO12536 and Micrococcus luteus IFO12992 and named FokI and MluI, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases
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