R4018

Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast

• Synonim(y): Transfer RNA, Yeast Phe tRNA, tRNA
• UNSPSC Code: 12352200
• NACRES: NA.55
• MDL number: MFCD00132200
• Form: lyophilized powder

Właściwości

• grade: Molecular Biology
• form: lyophilized powder
• mol wt: 25 kDa
• solubility: water: 2 mg/mL
• storage temp.: 2-8°C

Opis

General description

Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.

The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.

The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.

This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Application

Suitable for use:

• As a tRNA standard
• As a substrate in tRNA synthatase assays
• As a substrate in other reactions involving tRNAs

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).

Other Notes

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)