R0260
BamH I from Bacillus amyloliquefaciens H
Restriction Enzyme
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Molecular Biology
form
buffered aqueous glycerol solution
concentration
10,000 units/mL
shipped in
wet ice
storage temp.
−20°C
Application
BamHI is a DNA restriction endonuclease that is used in molecular biology applications to cleave DNA at the recognition sequence 5′-G/GATCC-3′ to generate 5′-cohesive termini.
Biochem/physiol Actions
Recognition sequence: 5′-G/GATCC-3′
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
Cutting results: a 2-10-fold Bam HI overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 60 °C for 15 minutes.
Star activity: To prevent star activity, avoid suboptimal reaction conditions containing low salt concentration, high glycerol (>5%) and high pH 8.0.
Physical form
Solution in 10 mM Tris-HCl, pH 7.4 , 1 mM EDTA, 1mM dithioerythritol, 300 mM KCl, 0.01% Polydocanol (v/v), 50% glycerol (v/v), at 4°C
Other Notes
Supplied with 10x Restriction Enzyme Buffer SB (B8781).
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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J H Ellis et al.
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G A Wilson et al.
Journal of molecular biology, 97(1), 123-125 (1975-09-05)
P Manivasakam et al.
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C Kessler et al.
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The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
Saqib H Ansari et al.
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β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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