Solutions prepared in water at 1 mg/mL may be stored in aliquots at -20°C for at least 6 months. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/076/p6621dat.pdf
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P6621
Phospholipase C from Bacillus cereus
≥200 units/mg protein
Synonim(y):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
250 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2080,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein
2080,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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Pozwól nam pomócform
lyophilized powder
Quality Segment
specific activity
≥200 units/mg protein
mol wt
23-27 kDa by SDS-PAGE
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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sustainability
Greener Alternative Product
solubility
H2O: soluble 1.0 mg/mL, faintly hazy to hazy
greener alternative category
storage temp.
−20°C
Gene Information
Bacillus cereus ATCC 10987 ... plC(2749087)
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling[1]. It is used to degrade inositol-containing phospholipids[2]. Phospholipase C from Bacillus, product P6621, has been used to study how flhF affects the export of PLC and other secretory virulence factors[3].
The enzyme from Sigma has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.[4]
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid[2]. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Physical form
Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate
Other Notes
One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
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Ta pozycja | |||
|---|---|---|---|
| specific activity ≥200 units/mg protein | specific activity ≥150 units/mg protein | specific activity 10-50 units/mg protein | specific activity ≥50 units/mg dry wt. |
| Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 23-27 kDa by SDS-PAGE | mol wt - | mol wt - | mol wt - |
| solubility H2O: soluble 1.0 mg/mL, faintly hazy to hazy | solubility - | solubility - | solubility aqueous buffer: soluble, water: soluble |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Is it ok to store this product in water at -20 C?
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Could you please provide the recommended storage temperature for the solution of this product?
1 answer-
The recommended storage for this product is at -20°C in working aliquots.
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