Przejdź do
MAK206
Fumarase Activity Colorimetric Assay Kit
Sufficient for 100 Colorimetric tests
Synonim(y):
Fumarate Hydratase Activity Colorimetric Assay Kit
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12352200
General description
The new Fumarase Assay Kit, MAK521, is now available! Fumarase (Fumarate Hydratase; EC 4.2.1.2) catalyzes the reversible hydration of fumarate to malate in the tricarboxylic acid (Krebs) cycle. The activity of fumarase is important as it produces reducing equivalents that drive the synthesis of ATP and maintains energy balance in aerobic metabolism. A deficiency of fumarase activity results in progressive encephalopathy.
Application
Suitable for the detection of Fumarase in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.
Fumarase Activity Colorimetric Assay Kit has been used to determine fumarase (FUM) activity.
Biochem/physiol Actions
The Fumarase Activity Colorimetric Assay Kit provides a simple and high-throughput ready procedure for measuring fumarase activity in a variety of tissues, cells, and isolated mitochondria. Fumarase activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of fumarase is the amount of enzyme that generates 1.0 mmole of NADH per minute at pH 9.5 at 37 °C.
Ta strona może zawierać tekst przetłumaczony maszynowo.
Tylko elementy zestawu
Numer produktu
Opis
- Fumarase Assay Buffer
- Fumarase Substrate
- Fumarase Enzyme Mix
- Fumarase Developer
- NADH Standard
- Fumarase Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Klasa składowania
8A - Combustible corrosive hazardous materials
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Powiązane treści
Instructions
Dieuwertje Augustijn et al.
PloS one, 14(6), e0218219-e0218219 (2019-06-27)
Climate change and the rising food demand provide a need for smart crops that yield more biomass. Recently, two Arabidopsis thaliana mutants with enhanced growth characteristics, VP16-02-003 and the VP16-05-014, were obtained by genome-wide reprogramming of gene expression, which led
Changes in specific protein degradation rates in Arabidopsis thaliana reveal multiple roles of Lon1 in mitochondrial protein homeostasis.
Li L, et al.
The Plant Journal, 89(3), 458-471 (2017)
Lei Li et al.
The Plant journal : for cell and molecular biology, 89(3), 458-471 (2016-10-12)
Mitochondrial Lon1 loss impairs oxidative phosphorylation complexes and TCA enzymes and causes accumulation of specific mitochondrial proteins. Analysis of over 400 mitochondrial protein degradation rates using 15 N labelling showed that 205 were significantly different between wild type (WT) and