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Merck

M8883

4-Methylumbelliferyl phosphate

phosphatase substrate, fluorogenic, ≥98% (HPLC), powder

Synonim(y):

4-Methylumbelliferyl-phosphoric acid

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Wybierz wielkość

100 MG

294,00 zł

250 MG

501,00 zł

1 G

1630,00 zł

5 G

5950,00 zł

294,00 zł

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C10H9O6P
Numer CAS:
3368-04-5
Masa cząsteczkowa:
256.15
NACRES:
NA.32
PubChem Substance ID:
24897292
UNSPSC Code:
12352204
EC Number:
222-137-7
MDL number:
MFCD00016969
Beilstein/REAXYS Number:
1687229

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Nazwa produktu

4-Methylumbelliferyl phosphate, phosphatase substrate

InChI key

BCHIXGBGRHLSBE-UHFFFAOYSA-N

InChI

1S/C10H9O6P/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14/h2-5H,1H3,(H2,12,13,14)

SMILES string

CC1=CC(=O)Oc2cc(OP(O)(O)=O)ccc12

Quality Level

200

assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL, clear to very slightly hazy, colorless

fluorescence

λex 319 nm; λem 384 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)

storage temp.

−20°C

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Ta pozycja
M8168M7008M3657
4-Methylumbelliferyl phosphate phosphatase substrate

Sigma-Aldrich

M8883

4-Methylumbelliferyl phosphate

4-Methylumbelliferyl phosphate disodium salt phosphatase substrate

Sigma-Aldrich

M8168

4-Methylumbelliferyl phosphate disodium salt

4-Methylumbelliferyl-β-D-xylopyranoside β-xylosidase substrate

Sigma-Aldrich

M7008

4-Methylumbelliferyl-β-D-xylopyranoside

4-Methylumbelliferyl α-D-mannopyranoside ≥97% (HPLC)

Sigma-Aldrich

M3657

4-Methylumbelliferyl α-D-mannopyranoside

assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

solubility

water: 25 mg/mL, clear to very slightly hazy, colorless

solubility

water: 50 mg/mL, clear, colorless

solubility

pyridine: 50 mg/mL, clear, colorless to faintly yellow

solubility

pyridine: 20 mg/mL, clear, colorless to faintly yellow

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

form

powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

fluorescence

λex 319 nm; λem 384 nm (pH 9.1), λex 360 nm; λem 449 nm (Reaction product)

fluorescence

λex 319 nm; λem 384 nm (pH 9.0), λex 360 nm; λem 449 nm (Reaction product)

fluorescence

-

fluorescence

λex 350 nm; λem 375 nm (pH 7.0), λex 360 nm; λem 449 nm (Reaction product)

General description

Fluorogenic substrate for phosphatases.

Application

4-Methylumbelliferyl phosphate has been used as a substrate for alkaline phosphatase in the peptide-binding assay.[1] It has also been used as a fluorogenic substrate in enzyme-linked immunosorbent assay (ELISA).[2][3]

Biochem/physiol Actions

4-Methylumbelliferyl phosphate serves as a fluorogenic substrate for calmodulin-dependent protein phosphatase and in kinetic studies of alkaline phosphatase. It is a substrate for alkaline phosphatase[4] in enzyme-linked immunosorbent assay (ELISA) procedures. 4-Methylumbelliferyl phosphate is seven times more sensitive than phenolphthalein monophosphate and 8-13 times more sensitive than with p-nitrophenyl phosphate when used in enzyme immunoassays for the detection of antibodies to human immunodeficiency viruses.
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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000534180
0000527916
0000534180
0000527916
0000517491

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Odwiedź Bibliotekę dokumentów

Yuka Hama et al.
Nutrients, 12(10) (2020-10-18)
Although folate deficiency was reported to be associated with hyperhomocysteinemia, influence of folate supplementation on cognition remains controversial. Therefore, we explored the effects of folate supplementation on the cognition and Homocysteine (Hcy) level in relatively short periods in patients with
Francesca Bertolini et al.
Journal of pharmaceutical sciences, 96(11), 2931-2944 (2007-08-21)
Oxidative damage to proteins, implicated amongst other in the etiology and progression of Parkinson's disease (PD) and Alzheimer's disease (AD), results in the loss of specific biological protein function. A simple, sensitive, and cost-effective fluorimetric test to assess the antioxidant
Laurent Gilardin et al.
Haematologica, 102(11), 1833-1841 (2017-07-29)
Acquired thrombotic thrombocytopenic purpura is a rare and severe disease characterized by auto-antibodies directed against "A Disintegrin And Metalloproteinase with Thrombospondin type 1 repeats, 13
N Bouaícha et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 40(11), 1677-1683 (2002-08-15)
Protein phosphatase inhibition assays currently used for the detection of cyanobacterial peptide hepatotoxins in drinking water require an enrichment step using C18 cartridges to achieve lower the detection limit. This paper describes a colorimetric and fluorometric protein phosphatase inhibition method
S A Rankin et al.
Journal of dairy science, 93(12), 5538-5551 (2010-11-26)
Standard practices for indirectly assessing the pasteurization status of milk products are primarily based on the thermal inactivation kinetics of the endogenous milk enzyme, alkaline phosphatase (ALP). This assessment provides an invaluable, if not required, tool for both regulatory and
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