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Merck

M7383

Sigma-Aldrich

Malic Dehydrogenase from porcine heart

≥400 units/mg protein (biuret), ammonium sulfate suspension

Synonim(y):

L-Malate: NAD+ oxidoreductase, MDH, Malate Dehydrogenase

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About This Item

Numer EC enzymu:
Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.54

Postać

ammonium sulfate suspension

aktywność właściwa

≥400 units/mg protein (biuret)

obecność zanieczyszczeń

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

temp. przechowywania

2-8°C

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Opis ogólny

Cytoplasmic Isozyme

Zastosowanie

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium.

Działania biochem./fizjol.

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms.

Definicja jednostki

One unit will convert 1.0 μmole of oxalacetate and β-NADH to L-malate and β-NAD per min at pH 7.5 at 25 °C.

Postać fizyczna

Suspension in 3.2 M (NH4)2SO4, 0.1 M KH2PO4, pH 7.0
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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K Smith et al.
Biochimica et biophysica acta, 884(1), 109-118 (1986-10-29)
The cationic surfactant, cetyl (hexadecyl) trimethylammonium bromide (CTAB), completely inactivates porcine heart cytoplasmic malate dehydrogenase (L-malate:NAD+ oxidoreductase, EC 1.1.1.37) at concentrations (of surfactant) which do not affect the activity of the mitochondrial isoenzyme. These concentrations are close to, or higher
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Nanogram amounts of soluble aminopeptidases, dehydrogenases, and esterases were detected by nondenaturing ultralow gelling point agarose-polyacrylamide gel electrophoresis (ULGA-PAGE). Cytosolic fractions from Frankia sp. were electrophoresed at 4 degrees C in the presence of Co2+, Zn2+, or Mg2+ ions. Then
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