M7257

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

• Synonim(y): α-D-Mannoside mannohydrolase
• Numer CAS: 9025-42-7
• NACRES: NA.54
• UNSPSC Code: 12352204
• EC Number: 232-794-1
• MDL number: MFCD00130495
• Numer WE: 3.2.1.24 (BRENDA, IUBMB)
• Specific activity: ≥15 units/mg protein (biuret)
• Biological source: Canavalia ensiformis

Właściwości

• biological source: Canavalia ensiformis
• Quality Segment: 200
• form: ammonium sulfate suspension
• specific activity: ≥15 units/mg protein (biuret)
• foreign activity: β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%
• storage temp.: 2-8°C
• SMILES string: [Zn+2]
• InChI: 1S/Zn/q+2
• InChI key: PTFCDOFLOPIGGS-UHFFFAOYSA-N

Opis

General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa. [1]

Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins. [2]

α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice. [3]

Liberates mannose from a variety of synthetic and natural α-mannosides.

α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:

• for the digestion of neutral glycans.
• to remove O-glycans from recombinant β-glucosidase
• for the inhibition of renal fibroblasts proliferation

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes. [1]

α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ and 0.1 mM zinc acetate, pH 7.5

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)