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M6320

Sigma-Aldrich

Monoclonal Anti-MAGE-1 antibody produced in mouse

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

Synonim(y):

Anti-Melanoma-associatiated antigen 1

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About This Item

Numer MDL:
MFCD03096998
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

MA454, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~46 kDa

reaktywność gatunkowa

human

opakowanie

antibody small pack of 25 μL

stężenie

~2 mg/mL

metody

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 5-10 μg/mL using total cell extract of SKML37 cells

izotyp

IgG1

numer dostępu UniProt

P43355

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MAGEA1(4100)

Opis ogólny

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen. MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28. Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

Specyficzność

The antibody does not recognize MAGE-2 and MAGE-3.

Immunogen

recombinant human MAGE-1.

Zastosowanie

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:
  • ELISA
  • immunoblotting
  • immunohistochemistry

Działania biochem./fizjol.

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC). Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Madhav V Dhodapkar et al.
Cancer immunity, 3, 9-9 (2003-07-24)
Cancer/testis (CT) antigens are expressed in several malignant tumors, but not in normal tissues except for testicular germ cells. The expression of CT antigenic proteins in malignant gammopathies has not been characterized. We examined the expression of a panel of
The MAGE Proteins: Emerging Roles in Cell
Cycle Progression, Apoptosis, and
Neurogenetic Disease
Barker PA and Salehi A
Journal of Neuroscience Research (2002)
Jiang Xiao et al.
World journal of gastroenterology, 10(13), 1849-1853 (2004-06-29)
To date, dozens of melanoma-associated antigens (MAGEs) have been identified and classified into 2 subgroups, I and II. Subgroup I consists of antigens which expression is generally restricted to tumor or germ cells, also named as cancer/testis (CT) antigen. Proteins
Clinical Significance of Melanoma Antigen-Encoding Gene-1 (MAGE-1) Expression and its Correlation with Poor Prognosis in Differentiated Advanced Gastric Cancer
Ogata MDK, et al.
Annals of Surgical Oncology (2011)
Y T Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1004-1008 (1994-02-01)
The human MAGE-1 gene encodes a melanoma peptide antigen recognized by autologous cytotoxic T lymphocytes. To produce antibodies against the MAGE-1 gene product, several approaches were taken. Three oligopeptides were synthesized based on predicted MAGE-1 amino acid sequences and were
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