K0253

Keratin from human epidermis

Name: Keratin from human epidermis
Brand: SIGMA

aqueous solution

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Informacje o tej pozycji

Numer CAS:

68238-35-7

UNSPSC Code:

12352202

NACRES:

NA.61

MDL number:

MFCD00131434

Form:

urea solution

Assay:

≥90% (SDS-PAGE)

Biological source:

human epidermis

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biological source

human epidermis

Quality Level

200

assay

≥90% (SDS-PAGE)

form

urea solution

technique(s)

ELISA: suitable

UniProt accession no.

P04264

storage temp.

2-8°C

Gene Information

human ... KRT1(3848)

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Ta pozycja	WH0003848M1	SAB4501653	SAB5500125
Sigma-Aldrich K0253 Keratin from human epidermis	Sigma-Aldrich WH0003848M1 Monoclonal Anti-KRT1 antibody produced in mouse	Sigma-Aldrich SAB4501653 Anti-Keratin 8 antibody produced in rabbit	Sigma-Aldrich SAB5500125 Anti-Keratin 17 antibody, Rabbit monoclonal
biological source human epidermis	biological source mouse	biological source rabbit	biological source rabbit
technique(s) ELISA: suitable	technique(s) indirect ELISA: suitable, western blot: 1-5 μg/mL	technique(s) ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000	technique(s) flow cytometry: 1:100, immunoblotting: 1:25, immunohistochemistry: 1:100
assay ≥90% (SDS-PAGE)	assay -	assay -	assay -
form urea solution	form buffered aqueous solution	form buffered aqueous solution	form -
UniProt accession no. P04264	UniProt accession no. P04264	UniProt accession no. P05787	UniProt accession no. Q04695
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

General description

Keratins are characteristic intermediate filament proteins of the epithelia. In human, keratin is classified into type I and type II. About 54 genes encodes for the keratin protein. The keratin 1 (KRT1) gene is mapped to human chromosome 12q13.13. It is the primary component of hair, skin and nails.

Application

Keratin from human epidermis has been used:

as an analyte and a cross-reactant in the antibody-immobilized chip for testing selectivity coefficients[1]
as a standard in extracellular matrix (ECM) protein in in vitro protein degradation assays.[2]
asa a standard in enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Keratins are important for the mechanical stability and integrity of epithelial cells and tissues. Some keratins also have regulatory functions and are involved in intracellular signaling pathways, e.g. protection from stress, wound healing, and apoptosis.[3] This diverse group of cytoskeletal scaffolding proteins form intermediate filament networks which provide structural support to keratinocytes that maintain the integrity of the skin. Expression of keratin genes is usually regulated by differentiation of the epidermal cells within the stratifying squamous epithelium.[4]

Physical form

Solution in 8 M urea, 50 mM Tris, 0.1 M β-mercaptoethanol and 0.1% sodium azide, pH 8.4

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Noninvasive in vitro and in vivo assessment of epidermal hyperkeratosis and dermal fibrosis in atopic dermatitis.

Jyh-Hong Lee et al.

Journal of biomedical optics, 14(1), 014008-014008 (2009-03-05)

Atopic dermatitis (AD) is characterized by hyperkeratosis of epidermis and fibrosis within dermis in chronic skin lesions. Thus far, the histology of skin lesions has been evaluated only by examination of excised specimens. A noninvasive in vivo tool is essential

Mutation of a major keratin phosphorylation site predisposes to hepatotoxic injury in transgenic mice.

N O Ku et al.

The Journal of cell biology, 143(7), 2023-2032 (1998-12-29)

Simple epithelia express keratins 8 (K8) and 18 (K18) as their major intermediate filament (IF) proteins. One important physiologic function of K8/18 is to protect hepatocytes from drug-induced liver injury. Although the mechanism of this protection is unknown, marked K8/18

Next-generation sequencing detection and characterization of a heterozygous novel splice junction mutation in the 2B domain of KRT1 in a family with diffuse palmoplantar keratoderma

Banerjee S, et al.

Experimental Dermatology, 24(2), 152-155 (2015)

Adenomatoid odontogenic tumor: a case report with immunohistological profile.

Francesca Angiero et al.

Anticancer research, 33(6), 2673-2677 (2013-06-12)

The adenomatoid odontogenic tumor (AOT) is an uncommon tumor of odontogenic origin, composed of odontogenic epithelium and characterized by slow but progressive growth. We report a rare case of AOT in an 18-year-old, who presented with a palpable bony-hard swelling

Tumor-promoting phorbol esters stimulate the proliferation of interleukin-3 dependent cells.

B B Hogans et al.

Journal of cellular physiology, 137(2), 346-352 (1988-11-01)

To determine whether activation of protein kinase C is involved in the proliferation of interleukin-3 (IL-3) -dependent cells, we examined the effect of tumor-promoting phorbol esters on the in vitro proliferation of the IL-3-dependent cell lines FD and DA-1. The

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Numer pozycji handlu globalnego

SKU	NUMER GTIN
K0253-1MG	04061826675557
K0253-5MG	04061833262931
K0253-10MG	04061833262924

Questions

a year ago

I need to ask about the supplied volume of this product. Also, can this product be used for fungal experimental studies as keratin is prepared in urea that might be toxic for cells?

1 answer
1. Technical Support
  
  11 months ago
  
  The volume of K0253 keratin derived from human epidermis is influenced by both the pack size and the lot-specific concentration. Here's a sample CofA for reference, which can be pulled from our website.
  https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/K0253-BULKSLCQ7891.pdf
  Also, keratin can be used for fungal experimental studies, but the presence of urea in its preparation may pose a concern depending on the concentration used. Urea can be cytotoxic at higher concentrations, potentially affecting fungal growth and experimental outcomes. To mitigate this, researchers often dialyze or extensively wash keratin preparations to remove residual urea. It is advisable to test the cytotoxicity of the prepared keratin on fungal cells before proceeding with experiments. Alternative keratin extraction methods that avoid urea may also be considered if cell viability is a major concern.
  K0253 is referenced in the study "Microbial Degradation of Native Keratin in Batch Fermentation".
  https://scialert.net/fulltext/fulltextpdf.php?pdf=ansinet/pjbs/1999/627-634.pdf
  
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