I9781

Importin β1 human

≥80% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), buffered aqueous glycerol solution

• Synonim(y): Importin β, Karyopherin β1, p97
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD03457282

Właściwości

• recombinant: expressed in E. coli (N-terminal histidine tagged)
• assay: ≥80% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: ~97 kDa by SDS-PAGE
• UniProt accession no.: Q14974
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KPNB1(3837)

Opis

General description

Importin β1 has 19 tandemly repeated HEAT (huntingtin, elongation factor 3 (EF3), protein phosphatase 2A (PP2A) and the yeast kinase TOR1) motifs.[1] The gene is mapped to human chromosome 17q21.[2] The protein shuttles between the cytoplasm and nucleus. It belongs to the importin β family of proteins.[3]

Biochem/physiol Actions

Import receptor, prototype of the nuclear transport receptor family, which interact directly with nuclear pore complex (NPC) and mediate nucleocytoplasmic transport.

Importin β, an import receptor, is a prototype of the nuclear transport receptor family, which comprises in human more than 20 proteins (90-180 kDa). These proteins interact directly with nuclear pore complex (NPC) and mediate nucleocytoplasmic transport. Importin β imports into the nucleus proteins carrying canonical nuclear localization signals (NLS) as well as UsnRNAs, which it binds via adaptor proteins, importin α and snurpotin-1 respectively. Importin β also binds directly, without adaptors, ribosomal proteins, Smad proteins, and virus derived proteins such as HIV Rev and Tat, that contain non classical NLS. In addition, Improtin b has been shown to be involved in the nuclear envelope assembly process.
The mechanism of importin β action in nuclear import can be demonstrated by the well-studied import of proteins containing classical NLS. Importin β forms a complex with Importin α, which, in turn, binds the cargo protein via its NLS. The Impβ/Impα/cargo complex translocates into the nucleus. When the complex reaches the nuclear site of the NPC, Ran-GTP binds the Impβ to form Impβ/Ran-GTP complex and released the Impα and the cargo protein. The Impβ/Ran-GTP complex is then exported to the cytoplasm where the complex dissociates upon hydrolysis of GTP to GDP, making Impβ ready for a new import cycle.

Physical form

Solution in 5% glycerol containing 20 mM Hepes, pH 7.5, 110 mM potassium-acetate, 2 mM magnesium-acetate, 0.5 mM EGTA, 0.1 mM ATP, 2 mM DTT, and protease inhibitors.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)