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H5000
Hexokinase from Saccharomyces cerevisiae
Type III, lyophilized powder, ≥25 units/mg protein (biuret)
Synonim(y):
ATP:D-Hexose-6-phosphotransferase, Hexokinase from yeast
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-611-5
MDL number:
Specific activity:
≥25 units/mg protein (biuret)
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Type III
form
lyophilized powder
specific activity
≥25 units/mg protein (biuret)
mol wt
dimer 110 kDa
foreign activity
glucose-6-phosphate dehydrogenase and phosphoglucose isomerase ≤10%
storage temp.
−20°C
Gene Information
bakers yeast ... HXK1(850614), HXK2(852639)
Biochem/physiol Actions
Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source.
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
The rate of phosphorylation varies with different hexoses (pH 7.5, 30 °C).
D-fructose KM: 0.33 mM
D-glucose KM: 0.12 mM
D-mannose KM: 0.05 mM
Yeast hexokinase exists as two similar isoforms, PI and PII (A and B), with isoelectric points of 5.25 and 4, respectively.
Molecular Weight: ~ 54 kDa (monomer)
~110 kDa (dimer)
Optimal pH: 7.5 to 9.0
Extinction Coefficient: E1% = 8.85 (PI) and 9.47 (PII) at 280 nm
Activators: Hexokinase requires Mg2+ ions (KM = 2.6 mM) for activity. Hexokinase is activated by catecholamines and related compounds.
Inhibitors: sorbose-1-phosphate, polyphosphates, 6-deoxy-6-fluoroglucose, 2-C-hydroxy-methylglucose, xylose, lyxose, and thiol reactive compounds (Hg2+ and 4-chloromercuribenzoate)
Physical form
Lyophilized powder containing approx. 15% sodium citrate
Other Notes
One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C, unless otherwise indicated below.
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Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Powiązane treści
Instructions
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PLoS genetics, 9(6), e1003563-e1003563 (2013-06-21)
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The Journal of cell biology, 203(4), 563-574 (2014-01-05)
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| H5000-250MG | 04061832924366 |