G6637

β-Galactose Dehydrogenase from Pseudomonas fluorescens

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

• Synonim(y): D-Galactose:NAD⁺ 1-oxidoreductase
• Numer CAS: 9028-54-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-837-4
• MDL number: MFCD00130622
• Numer WE: 1.1.1.48 (BRENDA, IUBMB)
• Specific activity: ≥50 units/mg protein (biuret)
• Assay: 0.5—2.0 mg protein/mL (biuret)
• Biological source: Pseudomonas fluorescens
• Recombinant: expressed in E. coli

Właściwości

• biological source: Pseudomonas fluorescens
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: 0.5—2.0 mg protein/mL (biuret)
• form: ammonium sulfate suspension
• specific activity: ≥50 units/mg protein (biuret)
• color: white
• suitability: suitable for enzyme test
• application(s): life science and biopharma
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: Pseudomonas fluorescens ... gdh(533113295)

Opis

Application

β-Galactose Dehydrogenase from Pseudomonas fluorescens has been used for competitive inhibition in lectin histochemistry.[1][2] It has also been used to measure the hydrolysis activity of Haloferax alicantei β-galactosidase on different disaccharides.[3]

Biochem/physiol Actions

β-galactose dehydrogenase catalyzes the oxidation of β-D-galactose to D-galactono-gammalactone.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, pH approx. 6.0

Other Notes

One unit will convert 1.0 μmole of D-galactose to D-galactonate per min at pH 8.6 at 25 °C.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)