E8399

Eotaxin-3 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonim(y): CCL26, SCYA26
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01867165

Właściwości

• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.25-1.0 mg
• mol wt: ~8.2 kDa
• packaging: pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: Q9Y258
• storage temp.: −20°C
• Gene Information: human ... CCL26(10344)

Opis

General description

Eotaxin-3, also known as CCL26 or SCYA26, is a human CC chemokine and a functional ligand for CC chemokine receptor 3.[1] This gene is localized to human chromosome 7q11.2, which codes for a protein of 71 amino acids.[2] It belongs to the small inducible cytokine subfamily A [3], which also includes eotaxin and eotaxin-2.[4]

Biochem/physiol Actions

Also known as CCL26 or SCYA26; a human CC chemokine and a functional ligand for CC chemokine receptor 3; mature recombinant human eotaxin-3 demonstrates chemotactic activity for eosinophils.

Eotaxin-3 is a potent attractant of eosinophils, and might be responsible for the accumulation of eosinophils in atopic diseases.[2] As it is involved in the recruitment of eosinophils in inflammatory diseases, it, along with its receptor, might have potential as therapeutic target in inflammatory disorders, such as asthma.[1] Demethylation results in increased expression of this gene, which in turn influences allergic reactions.[5] This gene is overexpressed in bullous pemphigoid (BP), which results in the accumulation of eosinophils in skin lesions, thus, contributing to the pathogenesis of BP.[4] Its expression is also elevated in the active lesions of patients with ulcerative colitis (UC) and Crohn′s disease (CD). Thus, it might play a role in the pathogenesis of UC, where its major source is colonic myofibroblasts.[6]

Eotaxin-3, also known as CCL26 or SCYA26, is a human CC chemokine and a functional ligand for CC chemokine receptor 3. Mature recombinant human eotaxin-3 has a molecular mass of approximately 8.2 kDa and demonstrates chemotactic activity for eosinophils.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 1.25 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to induce chemotaxis of rat Y3 cells stably expressing hCCR3.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H319,H332
• pcodes: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Irrit. 2
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable