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1 ML
3110,00 zł
5 X 1 ML
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Informacje o tej pozycji
NACRES:
NA.56
UNSPSC Code:
12352203
Technique(s):
affinity chromatography: suitable, immunoprecipitation (IP): suitable
Species reactivity:
-
Application:
IP, affinity chromatography
Citations:
143
Przejdź do
Pomoc techniczna
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Pozwól nam pomócshelf life
1 yr at 2‑8 °C
analyte chemical class(es)
proteins (c-Myc)
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable
capacity
~0.05 mg/mL binding capacity
storage temp.
2-8°C
1 of 4
Ta pozycja | |||
|---|---|---|---|
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable | technique(s) affinity chromatography: suitable, immunoprecipitation (IP): suitable |
| shelf life 1 yr at 2‑8 °C | shelf life 1 yr at 2‑8 °C | shelf life - | shelf life - |
| analyte chemical class(es) proteins (c-Myc) | analyte chemical class(es) proteins (hemagglutinin) | analyte chemical class(es) proteins | analyte chemical class(es) proteins (c-Myc) |
| capacity ~0.05 mg/mL binding capacity | capacity ≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein) | capacity ≥0.6 mg/mL, gel binding capacity | capacity ≥10 nmol/mL binding capacity (c-Myc tagged fusion protein), ≥7 nmol/mL elution capacity (c-Myc tagged fusion protein) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
EZ view Red Anti--c-Myc Affinity Gel is a resin that consists of the Anti-c-Myc rabbit antibody, covalently bonded to 4% Agarose beads. The affinity gel is used to bind c-Myc fusion proteins to samples, such as cell lysates and tissue, for purification of c-Myc-tagged proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.
Agarose beads bind at N-terminal, Met-N-terminal and C-terminal c-Myc fusion proteins from E. coli and mammalian cells.
Application
EZview™ Red Anti-c-Myc Affinity Gel is suitable for:
- SUMOylation assay[1]
- Affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduce the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.
Features and Benefits
- Increased visibility - Red color reduces risk of incidental aspiration
- Improved recovery of target protein by reduced accidental loss
- Higher reproducibility - More consistent yields
Physical form
1:1 (v/v) suspension in PBS containing 50% glycerol and 0.0015% Kathon CG/IPCII as an antimicrobial preservative
Other Notes
The affinity gel contains affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to beaded agarose. The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein.
Legal Information
EZview is a trademark of Sigma-Aldrich Co. LLC
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Klasa składowania
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| E6654-1ML | 04061838087379 |
| E6654-5X1ML | 04061833604625 |
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