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Merck

E1250

Elastase from porcine pancreas

Type I, ≥4.0 units/mg protein

Synonim(y):

Elastase from hog pancreas, Pancreatopeptidase E

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Wybierz wielkość

10 MG

468,00 zł

25 MG

846,00 zł

100 MG

1460,00 zł

50 MG

1660,00 zł

500 MG

6090,00 zł

468,00 zł

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Informacje o tej pozycji

Numer CAS:
39445-21-1
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
254-453-6
MDL number:
MFCD00130998
Numer WE:
3.4.21.36 (BRENDA, IUBMB)
Specific activity:
≥4.0 units/mg protein
Biological source:
Porcine pancreas
Concentration:
0.5-15.0 mg/mL in water

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biological source

Porcine pancreas

type

Type I

form

suspension

specific activity

≥4.0 units/mg protein

mol wt

25.9 kDa

contains

0.1% thymol

concentration

0.5-15.0 mg/mL in water

foreign activity

trypsin ≤50 BAEE units/mg protein

storage temp.

2-8°C

Quality Level

200

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Ta pozycja
E0127324682E0258
Elastase from porcine pancreas Type I, ≥4.0 units/mg protein

Sigma-Aldrich

E1250

Elastase from porcine pancreas

Elastase from porcine pancreas Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein

Sigma-Aldrich

E0127

Elastase from porcine pancreas

Elastase, Porcine Pancreas, High Purity, Crystallized Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

Sigma-Aldrich

324682

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase from porcine pancreas Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)

Sigma-Aldrich

E0258

Elastase from porcine pancreas

biological source

Porcine pancreas

biological source

-

biological source

-

biological source

Porcine pancreas

specific activity

≥4.0 units/mg protein

specific activity

≥4.0 units/mg protein

specific activity

≥25 units/mg protein

specific activity

≥4.0 units/mg protein (biuret)

form

suspension

form

lyophilized powder

form

lyophilized

form

lyophilized powder

concentration

0.5-15.0 mg/mL in water

concentration

-

concentration

-

concentration

-

mol wt

25.9 kDa

mol wt

25.9 kDa

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Elastase from porcine pancreas has been used:
  • to induce abdominal aortic aneurysm (AAA)
  • to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice
  • to digest aortas for aortic smooth muscle cells (SMC) isolation
  • as a positive control of proteolytic digestion

Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase.
The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development.[1] It has also been used during the isolation of type II pneumocytes from human lungs.[2]

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.

Packaging

Package size based on protein content

Preparation Note

2× crystallized

Other Notes

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certyfikaty analizy (CoA)

Lot/Batch Number
0000381849
0000398274
0000392898
0000392896
0000390817

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Demetrios Moris et al.
Current vascular pharmacology, 16(2), 168-178 (2017-02-06)
Abdominal aortic aneurysm (AAA) formation is associated with by inflammation and matrix degradation. This study tested the hypothesis that calprotectin, a novel biomarker for inflammation, as well as established biomarkers such as C-reactive protein (CRP) and matrix metalloproteinase- 9 (MMP-9)
A C Cunningham et al.
Journal of cell science, 107 ( Pt 2), 443-449 (1994-02-01)
Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli.
Andreas Giraud et al.
Cardiovascular research, 113(11), 1364-1375 (2017-06-06)
Abdominal aortic aneurysm (AAA), frequently diagnosed in old patients, is characterized by chronic inflammation, vascular cell apoptosis and metalloproteinase-mediated extracellular matrix destruction. Despite improvement in the understanding of the pathophysiology of aortic aneurysm, no pharmacological treatment is yet available to
Michel P Bergoeing et al.
Journal of vascular surgery, 45(6), 1217-1227 (2007-04-03)
The development of abdominal aortic aneurysms (AAA) is presumed to result from multiple genetic and environmental factors, with exposure to tobacco smoke the single largest known factor predisposing to aneurysm growth. We have attempted to adapt the elastase-perfused animal model
Júlia Benini Kohler et al.
Biology open, 8(4) (2019-04-12)
Macrophages play a pivotal role in the development of emphysema and depending on the microenvironment stimuli can be polarized into M1- or M2-like macrophage phenotypes. We compared macrophage polarizations in cigarette smoke (CS)- and porcine pancreatic elastase (PPE)-induced emphysema models.
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Protokoły

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Test enzymatyczny elastazy (EC 3.4.21.36)

Test enzymatyczny elastazy (EC 3.4.21.36), ciągłe spektrofotometryczne oznaczanie szybkości.

Questions

  1. What is the difference between Type I and Type IV Elastase?

    1 answer

    1. The major differences between the different types of Elastase are as follows:

      E1250: Type I, aqueous suspension, >4 units/mg protein, <50 units/mg protein Trypsin. Contains Thymol as an antimicrobial, which is toxic to cells.

      E6883: Type IIA, discontinued

      E0127: Type III, lyophilized powder, >4 units/mg protein, <200 units/mg protein Trypsin.

      E0258: Type IV, lyophilized powder, >4 units/mg protein, <50 units/mg protein Trypsin, further purification of E0127.

      E7885: Bioreagent, lyophilized powder, suitability tested for use in cell culture, >4 units/mg protein, <50 units/mg protein Trypsin.

      45125: Lyophilized powder, >6.4 units/mg protein, not tested for Trypsin impurity.

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