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E1131
Exonuclease III from Escherichia coli BE25 /psGR3
buffered aqueous glycerol solution
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
EC Number:
232-912-1
MDL number:
grade
Molecular Biology
form
buffered aqueous glycerol solution
mol wt
28 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xthA(946254)
General description
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
Application
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
Other Notes
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Ming Luo et al.
Chemical communications (Cambridge, England), 48(59), 7416-7418 (2012-06-21)
An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.
Peng Hu et al.
Biosensors & bioelectronics, 34(1), 83-87 (2012-03-03)
We report here a graphene oxide (GO)-based fluorescent aptasensor for adenosine detection by employing exonuclease III (Exo III) as a signal amplifying element. In the absence of adenosine, the adenosine aptamers hybridized with the complementary DNA (cDNA), and the Exo
Xiaofeng Wei et al.
Chemical communications (Cambridge, England), 48(49), 6184-6186 (2012-05-17)
Based on the specific folic acid-folate receptor (FA-FR) interaction, macromolecular FR can bind with FA-linked DNA-small molecule chimeras, which can prevent enzymolysis by exonuclease III (Exo III), enabling a novel fluorescence biosensor for FR to be developed using quinaldine red