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D9909
Dextran Sucrase from Leuconostoc mesenteroides
lyophilized powder, ≥100 units/mg protein
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 10 units | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 1880,00 zł |
Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
bacterial (Leuconostoc mesenteroides)
1880,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bacterial (Leuconostoc mesenteroides)
Quality Level
form
lyophilized powder
specific activity
≥100 units/mg protein
composition
Protein, ~15% Lowry
solubility
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
storage temp.
−20°C
General description
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.
Application
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production
- in the enzymatic synthesis of dextran nanoparticles at various pH range
- in the immobilization with hydroxyapatite for dextran production
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
Biochem/physiol Actions
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[1] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[2] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[3]
Physical form
Lyophilized powder containing dextran, MES buffer salts and CaCl2
Analysis Note
Chromatographically purified
Other Notes
One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.
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Ta pozycja | |||
|---|---|---|---|
| biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) | biological source bacterial (Leuconostoc mesenteroides) |
| specific activity ≥100 units/mg protein | specific activity - | specific activity - | specific activity - |
| form lyophilized powder | form powder | form powder | form powder |
| solubility H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow | solubility H2O: soluble 20 mg/mL | solubility H2O: 100 mg/mL, clear, colorless | solubility H2O: 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Ah-Rum Yi et al.
Journal of microbiology and biotechnology, 19(8), 829-835 (2009-09-08)
A dextransucrase (LcDS) gene from Leuconostoc citreum HJ-P4 has been amplified and cloned in E. coli. The LcDS gene consists of 4,431 nucleotides encoding 1,477 amino acid residues sharing 63-98% of amino acid sequence identities with other known dextransucrases from
Hongbin Zhang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 25(12), 2022-2028 (2010-04-01)
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The
Marie-Sophie Bounaix et al.
FEMS microbiology letters, 311(1), 18-26 (2010-08-21)
The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibaria
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| D9909-10UN | 04061833591352 |