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D0428

1 kb DNA Ladder

for DNA electrophoresis

Synonim(y):

1kb gel ladder, 1kb gel marker, 1kb ladder for gel electrophoresis, DNA gel marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

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Do Państwa/SKUDostępnośćCena netto
1 vial
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1620,00 zł

Informacje o tej pozycji

NACRES:
NA.25
UNSPSC Code:
41105335

1620,00 zł

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Quality Segment

100, 200

form

liquid

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR™ Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.[1]

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries
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Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  

  1. Does this product have biological origin? Or is the DNA synthetically formulated?

    1 answer

    1. This product has historically been synthetically produced. However, it is best to refer to the lot-specific Certificate of Origin, as the method of manufacture and origin of future lots may be subject to change. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab or click on the link below to request a lot-specific Certificate of Origin:
      https://www.sigmaaldrich.com/product/sigma/d0428#product-documentation

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  2. how to use this product. in what ratio we have to add dye in this.

    1 answer

    1. This product has been tested successfully at 2 µL, 5µL, and 7 µL., however for general use, a starting volume of 5 µL is recommended. Dilute 5 µL volume of D0428 with 25 µL of gel loading buffer. Product G2526 is used for testing purposes. For best results, a 0.75% gel is recommended.
      See the link below to Gel Loading Buffer, G2526:
      https://www.sigmaaldrich.com/product/sigma/g2526

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