C4405
Choline Oxidase from Arthrobacter globiformis
lyophilized powder, 8-20 units/mg solid
Synonim(y):
Choline: oxygen 1-oxidoreductase
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Informacje o tej pozycji
Numer CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-840-0
MDL number:
Specific activity:
8-20 units/mg solid
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Pozwól nam pomócform
lyophilized powder
Quality Level
specific activity
8-20 units/mg solid
mol wt
71 kDa by SDS-PAGE, 83 kDa by gel filtration
storage temp.
−20°C
1 of 4
Ta pozycja | |||
|---|---|---|---|
| specific activity 8-20 units/mg solid | specific activity ≥10 units/mg solid | specific activity ≥12 units/mg protein | specific activity ≥50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form powder | form lyophilized powder |
| mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration | mol wt ~95 kDa | mol wt - | mol wt 64 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
Research area: Cell signaling. Choline oxidaseis a flavoprotein and belongs to the glucose methanol choline(GMC)-oxidoreductase family.[1]
Application
Choline oxidase from Sigma has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection.[2]Choline oxidase from Arthrobacterglobiformis is suitable for the preparation of amperometric biosensors.
Choline Oxidase from Arthrobacter globiformis has been used:
- for enzymatic determination of choline (Cho) and phosphocholine (P-Cho)
- in sphingomyelin assay to determine the concentration of sphingomyelin by measuring free choline
- in enzyme coating for the fully-assembled recording device for electrochemical measurements
Biochem/physiol Actions
Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine.[3] The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.[4]
Other Notes
One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
A multi-site array for combined local electrochemistry and electrophysiology in the non-human primate brain
Disney AA, et al.
Journal of Neuroscience Methods, 255, 29-37 (2015)
Choline import into chloroplasts limits glycine betaine synthesis in tobacco: analysis of plants engineered with a chloroplastic or a cytosolic pathway
Nuccio ML, et al.
Metabolic engineering, 2(4), 300-311 (2000)
Rungaroon Waditee et al.
The Journal of biological chemistry, 278(7), 4932-4942 (2002-12-06)
Glycine betaine (N,N,N-trimethylglycine) is an important osmoprotectant and is synthesized in response to abiotic stresses. Although almost all known biosynthetic pathways of betaine are two-step oxidation of choline, here we isolated two N-methyltransferase genes from a halotolerant cyanobacterium Aphanothece halophytica.
Shufen Li et al.
Plant, cell & environment, 34(11), 1931-1943 (2011-06-30)
Tomato (Lycopersicon esculentum cv. 'Moneymaker') was transformed with a codA gene, from Arthrobacter globiformis, for choline oxidase that had been modified to allow targeting to both chloroplasts and the cytosol. Glycinebetaine (GB) accumulated in seeds of transformed plants up to
Fan Fan et al.
Journal of the American Chemical Society, 127(7), 2067-2074 (2005-02-17)
Choline oxidase catalyzes the four-electron oxidation of choline to glycine betaine, with betaine aldehyde as an intermediate. In this study, primary deuterium and solvent kinetic isotope effects have been used to elucidate the mechanism for substrate oxidation by choline oxidase
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Instructions
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| C4405-500UN | 04061833492116 |
| C4405-100UN | 04061833492109 |
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