C30

Catalase from bovine liver

aqueous suspension, 10,000-40,000 units/mg protein

• Synonim(y): Bovine hepatocatalase, H₂O₂:H₂O₂ oxidoreductase
• Numer CAS: 9001-05-2
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• Numer WE: 1.11.1.6 (BRENDA, IUBMB)
• MDL number: MFCD00081483
• Specific activity: 10,000-40,000 units/mg protein
• Biological source: bovine liver
• Concentration: 20-50 mg/mL

Właściwości

• biological source: bovine liver
• Quality Segment: 200
• form: aqueous suspension
• specific activity: 10,000-40,000 units/mg protein
• mol wt: tetramer ~250 kDa
• concentration: 20-50 mg/mL
• technique(s): GC/MS: suitable
• isoelectric point: 5.4
• UniProt accession no.: P00432
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: O(CC)C(=O)c1ccc(cc1)O
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• Gene Information: cow ... CAT(280743)

Opis

General description

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Catalase from bovine liver contains 506 residues. It is a tetramer and each monomer corresponds to a molecular weight of 61 kDa. The active site in each momomer comprises nicotinamide adenine dinucleotide phosphate (NADPH) and iron binding region.

Research area: Cell Signaling

Application

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

• to prepare H₂O₂-O₂ based biocathode for applications in glucose biofuel cells
• to study the kinetic properties and storage stability of catalase immobilized on to florisil
• in glutathione-mediated superoxide generation in an aqueous solution

Catalase from bovine liver is used for the following applications:

• Dielectrophoretic field-flow fractionation (DEP-FFF): It is a chromatographic method in which cell elution times reflect the positions of cells in a hydrodynamic flow profile under the control of sedimentation, DEP and hydrodynamic lift forces, FSED, FDEP and FHDL, respectively. The DEP-FFF buffer consists of catalase along with other ingrediants.[1][2]
• GC-MS analyses.[3]
• Deionization.[4]
• GC-FID analysis.5
• Functional analysis of blood vessel.[5]

Catalase from bovine liver has been used for measuring the hydrogen peroxide content in cancer tissue homogenates. It has also been used to test the effect of organophosphate insecticide chlorpyrifos-ethyl (CE) [0,0-diethyl 0 (3,5,6-trichloro-2-pyridyl) phosphorothioate] on its enzyme activity.

Biochem/physiol Actions

Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H₂O₂ molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

This product doesn′t need any activators, but it is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogens bromide, 2-mercaptoethanol, dithiothreitol, dianisidnie and nitrate.

Preparation Note

This product is a crystalline suspension in water containing 0.1% thymol with activity of 10,000-40,000 units/mg.

Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Other Notes

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Disclaimer

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Klasa składowania: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US), type N95 (US)