B9304

Anti-Bcl-x antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonim(y): Anti-BCL2L1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00675212
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, IHC (p), WB
• Citations: 9

Właściwości

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 26-30 kDa
• species reactivity: mouse, human, rat
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human colon adenocarcinoma sections, microarray: suitable, western blot: 1:2,000 using human Burkit lymphoma Raji cell lysate
• UniProt accession no.: Q07817
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... BCL2L1(598) ; mouse ... Bcl2l1(12048) ; rat ... Bcl2l1(24888)

Opis

General description

Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x, long isoform (Bcl-x) gene is mapped to human chromosome 20q11.21. BCL2L1 belongs to the Bcl-2 family. The bcl-x gene on alternative splicing leads two distinct proteins. It comprises multiple Bcl-2 homology (BH3) domains. Bcl-xL is expressed in the adult brain.(10)

Immunogen

synthetic peptide corresponding to the N-terminal of human Bcl-x (amino acids 2-20) conjugated to BSA. This sequence is identical in human and mouse Bcl-x_S, Bcl-x_L, Bcl-Xβ and Bcl-Xγ isoforms and is highly conserved in rat Bcl-x_S and Bcl-x_L (single amino acid substitution).

Application

Anti-Bcl-x antibody produced in rabbit may be used in immunoblotting[1] and immunohistochemistry.[2]

Biochem/physiol Actions

Bcl-2-like protein 1 (BCL2L1) or Bcl-2-related gene x arelong isoforms (Bcl-x) of proteins that are involved in regulating apoptosis. Similar to Bcl-2, Bcl-xL can inhibit cell death upon growth factor removal, when transfected in an IL-3 dependent cell line. Bcl-xL can form ion channels in biological membranes and may maintain cell survival by regulating the permeability of intracellular membranes. Bcl-xL may bind to apoptotic protease activating factor 1 (Apaf-1) preventing it from activating caspase 9. Like Bcl-2, Bcl-x activity is regulated by specific phosphorylation of a 60-amino acid regulatory loop region. Bcl-xL has been shown to regulate cell response to oxidative stress, cellular resistance to DNA damage-induced apoptosis by chemotherapeutic agents and ionizing radiation, and favors neurite outgrowth.

The antibody reacts with Bcl-x_L.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in "frost-free" freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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