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Dokumenty

A9979

Sigma-Aldrich

Anti-Aly antibody, Mouse monoclonal

clone 11G5, purified from hybridoma cell culture

Synonim(y):

Anti-mREF1-I

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About This Item

Numer MDL:
MFCD07785404
Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

mouse

białko sprzężone

unconjugated

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

11G5, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

antigen ~30 kDa

reaktywność gatunkowa

Xenopus, human

stężenie

~2 mg/mL

metody

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extract of HeLa cells

izotyp

IgG1

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... THOC4(10189)

Opis ogólny

Aly is a nuclear chaperone protein that may modulate DNA binding, mRNA synthesis, and dimerization of basic region-leucine zipper proteins. Mouse Monoclonal Anti-Aly antibody recognizes human and Xenopus Aly.
Anti-Aly antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 11G5 hybridoma produced by the fusion of mouse myeloma cells (SP2/O) and splenocytes from BALB/c mice immunized with recombinant human Aly/REF protein. Aly is also known as mREF1-I which is an RNA-binding protein that contains an RNA-binding domain and is recruited to the mRNA-protein complex (mRNP) complexes during the mRNA splicing.

Immunogen

recombinant human Aly/REF protein.

Zastosowanie

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Co-immunoprecipitation and western blot studies were performed in Hela cells using a monoclonal mouse anti-Aly antibody. The results indicated that Aly which was observed as a 30 kDa band interacted with the Herpes Simplex Virus I protein, ICP27.
Mouse Monoclonal Anti-Aly antibody can be used for immunocytochemistry, immunoprecipitation, indirect ELISA, microarray and western blot (0.5-1.0μg/ml) assays.

Działania biochem./fizjol.

Excess of recombinant expression of Aly in the cells increases both the rate and efficiency of spliced and unspliced mRNA export (in vivo) from the nucleus to the cytoplasm. Aly is dissociated from the mRNAs after the export to the cytoplasm.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Messenger-RNA-binding proteins and the messages they carry
Dreyfuss G, et al.
Nature Reviews in Molecular and Cell Biology, 3(3), 195-195 (2002)
Amir Mor et al.
Nature microbiology, 1(7), 16069-16069 (2016-08-31)
Influenza A virus is a human pathogen with a genome composed of eight viral RNA segments that replicate in the nucleus. Two viral mRNAs are alternatively spliced. The unspliced M1 mRNA is translated into the matrix M1 protein, while the
The protein Aly links pre-messenger-RNA splicing to nuclear export in metazoans
Zhou Z, et al.
Nature, 407(6802), 401-401 (2000)
Ella Alkalay et al.
Cells, 9(9) (2020-08-29)
RNA-binding proteins, particularly splicing factors, localize to sub-nuclear domains termed nuclear speckles. During certain viral infections, as the nucleus fills up with replicating virus compartments, host cell chromatin distribution changes, ending up condensed at the nuclear periphery. In this study
Johnathan Cooper-Knock et al.
Brain : a journal of neurology, 137(Pt 7), 2040-2051 (2014-05-29)
GGGGCC repeat expansions of C9orf72 represent the most common genetic variant of amyotrophic lateral sclerosis and frontotemporal degeneration, but the mechanism of pathogenesis is unclear. Recent reports have suggested that the transcribed repeat might form toxic RNA foci that sequester
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