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A7979

Bovine Serum Albumin solution

35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonim(y):

Albumin solution from bovine serum, BSA solution

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Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
33-37% total protein basis (UV)
Form:
liquid
Technique(s):
blocking: suitable, cell culture | mammalian: suitable
Impurities:
≤1000 EU/mL Endotoxin level
Concentration:
35% in DPBS
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biological source

bovine

Quality Segment

description

endotoxin tested

sterility

sterile-filtered

product line

BioXtra

assay

33-37% total protein basis (UV)

form

liquid

origin

USA origin

concentration

35% in DPBS

technique(s)

blocking: suitable, cell culture | mammalian: suitable

impurities

≤1000 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma Test by Barile On raw material, none detected

shipped in

ambient

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
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Ta pozycja
A9576A9205A3156
technique(s)

blocking: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, electrophoresis: suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

assay

33-37% total protein basis (UV)

assay

28-32% total protein basis (UV)

assay

28-32% protein basis (biuret)

assay

≥99% (agarose gel electrophoresis)

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

Quality Level

400

Quality Level

300

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

solution

form

lyophilized powder


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Klasa składowania

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Questions

1–4 of 4 Questions  
  1. Is it suitable for biotin/streptavidin-mediated detection systems?

    1 answer
    1. This product is not tested for biotin levels or suitability in biotin/streptavidin-mediated detection systems.

      Helpful?

  2. IS IT FREE FROM CALCIUM AND MAGNESIUM?

    1 answer
    1. This product is prepared in Dulbeccos Phosphate Buffered Saline without calcium and magnesium.

      Helpful?

  3. What is the concentration of this product?

    1 answer
    1. As shown in the DESCRIPTION section of the Product Detail Page, the BSA concentration for Product A7979, is 35% w/v in DPBS.

      Helpful?

  4. Hi, We have used Bovine serum albumin (A6003) and prepared a 10% BSA solution in buffer. There are also several BSA solutions available, but I can't see any information what BSA solid that was used (Product/Cat number). Has any the same origin?

    1 answer
    1. Product A6003 is a fatty acid-free BSA powder.

      See the links below to review the fatty acid-free BSA solutions currently prepared from this material.
      A1595, 10% BSA Solution, Fatty acid-free, in DPBS
      https://www.sigmaaldrich.com/product/sigma/a1595

      A9205, 30% BSA Solution, Fatty acid-free, in DPBS
      https://www.sigmaaldrich.com/product/sigma/a9205

      Helpful?

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