94072246

RPMI 7932 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072246, human lung effusion, Epithelial-like morphology

• UNSPSC Code: 41106514
• Biological source: human lung (effusion)
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial-like

Właściwości

• biological source: human lung (effusion)
• Quality Segment: 100
• form: liquid
• packaging: tube of 5 μg 94072246-DNA-5UG, pkg of vial of cells 94072246-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Plasminogen activator
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer

Opis

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from the pleural effusion of a 36 year old female with malignant melanoma. Cell line produces plasminogen activator. This cell line was found to be indistinguishable from cell lines COLO 699N & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.

Human pleural effusion from a patient with malignant melanoma

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-6x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable