77160

Pepsin from porcine gastric mucosa

powder, slightly beige, ≥500 U/mg

• Synonim(y): Pepsin A, Pepsin from hog stomach
• Numer CAS: 9001-75-6
• UNSPSC Code: 12352204
• eCl@ss: 42010127
• EC Number: 232-629-3
• NACRES: NA.54
• MDL number: MFCD00081840
• Numer WE: 3.4.23.1 (BRENDA, IUBMB)
• Specific activity: ≥500 U/mg

Właściwości

• form: powder
• Quality Segment: 200
• specific activity: ≥500 U/mg
• mol wt: 35 kDa
• color: slightly beige
• UniProt accession no.: P00791
• storage temp.: 2-8°C
• Gene Information: pig ... LOC396892(396892)

Opis

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

One unit corresponds to the amount of enzyme which increases the absorbance at 280 nm by 0.001 per minute at pH 2.0 and 37 °C (Hemoglobin, Catalog No. 51290, as substrate); 15,000 absorbance units as described above are equivalent to ∼1 Bergmeyer unit. One Bergmeyer unit is the amount of enzyme which hydrolyzes 1 μmole Acetyl-L-phenyl-3,5-diiodo-L-tyrosine at pH 2.0 and 37 °C.

Peptide synthesis,citationcitation Review.citation

Peptide synthesis,[1] Review.

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Informacja o środkach bezpieczeństwa

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves