50935

Guanidine hydrochloride

BioUltra, ≥99.5% (AT)

• Synonim(y): Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Wzór liniowy: NH₂C(=NH)NH₂ · HCl
• Numer CAS: 50-01-1
• Masa cząsteczkowa: 95.53
• EC Number: 200-002-3
• UNSPSC Code: 12352200
• PubChem Substance ID: 24873454
• Beilstein/REAXYS Number: 3591990
• MDL number: MFCD00013026

Właściwości

• product line: BioUltra
• assay: ≥99.5% (AT)
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.05% (as SO₄)
• loss: ≤0.05% loss on drying, 20 °C (HV)
• pH: 4.5-6.0 (25 °C, 6 M in H₂O)
• mp: 180-185 °C (lit.)
• solubility: H₂O: 6 M at 20 °C, clear, colorless
• density: 1.3 g/cm³ (lit.)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 6 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.03, λ: 280 nm A_max: 0.02
• SMILES string: Cl[H].NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N

Opis

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Other Notes

In the extraction and fractionation by cesium sulfate density gradient centrifugation of bovine nasal cartilage proteoglycan[1]; Induces subunit dissociation and unfolding of bovine liver glutamate dehydrogenase[2]; Dissociation of apolipoproteins of insect lipophorin[3]; Denaturation curve[4].

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Informacja o środkach bezpieczeństwa

• pictograms: GHS07
• signalword: Warning
• hcodes: H302 + H332,H315,H319
• pcodes: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves