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41176

Anti-Rabbit IgG - Atto 633 antibody produced in goat

1 mg/mL IgG

Synonim(y):

Atto 633 [goat-Anti-rabbit IgG]

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Informacje o tej pozycji

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
Atto 633 conjugate
Clone:
polyclonal
Application:
IHC
Citations:
9

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biological source

goat

conjugate

Atto 633 conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

rabbit

concentration

1 mg/mL IgG

technique(s)

immunohistochemistry: 1:200

fluorescence

λex 636 nm; λem 650 nm in PBS

storage temp.

−20°C

target post-translational modification

unmodified

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A381277671A0545
Sigma-Aldrich

Sigma-Aldrich

41176

Anti-Rabbit IgG - Atto 633 antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3812

Anti-Rabbit IgG (whole molecule)–Alkaline Phosphatase antibody produced in goat

Sigma-Aldrich

Sigma-Aldrich

77671

Anti-Rabbit IgG - Atto 594 antibody produced in goat

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat affinity isolated antibody

Sigma-Aldrich

A0545

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

technique(s)

immunohistochemistry: 1:200

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

-

technique(s)

direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

-

antibody form

affinity isolated antibody

conjugate

Atto 633 conjugate

conjugate

alkaline phosphatase conjugate

conjugate

Atto 594 conjugate

conjugate

peroxidase conjugate

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

biological source

goat

biological source

goat

biological source

-

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

General description

Anti-rabbit IgG (whole molecule) (Sigma, R4880) is developed in goat using purified rabbit IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-rabbit IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. Goat anti-rabbit is conjugated to Atto 633 NHS (Abs.max. 629 nm; Em.max. 657 nm), (Cat. No. 01464), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

Immunogen

purified rabbit IgG

Application

Goat anti-rabbit IgG can be used for immunohistochemical applications at a dilution of 1:200.

Analysis Note

unconjugated dye ≤5% of total fluorescence

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Numer produktu
Opis
Cennik

Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
BCCJ9566
BCBX5989
BCBW7606
BCBS0037V
BCBP2607V

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Jeśli potrzebujesz konkretnej wersji, możesz wyszukać konkretny certyfikat według numeru partii lub serii.

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Maëva Clerte et al.
Journal of the American Society of Echocardiography : official publication of the American Society of Echocardiography, 26(12), 1465-1473 (2013-09-03)
When activated by the sympathetic nervous system, brown adipose tissue (BAT) increases energy expenditure to produce heat. Augmenting BAT mass or increasing BAT activation could potentially be used to decrease obesity. Noninvasive methods to detect and monitor BAT mass are
Fanny Dussert et al.
Frontiers in toxicology, 3, 636976-636976 (2022-03-18)
Quantum dots (QDs) are colloidal fluorescent semiconductor nanocrystals with exceptional optical properties. Their widespread use, particularly in light-emitting diodes (LEDs), displays, and photovoltaics, is questioning their potential toxicity. The most widely used QDs are CdSe and CdTe QDs, but due
Huildore Bommanna Ranjitha et al.
Journal of cell science, 134(5) (2020-06-03)
Foot-and-mouth disease virus (FMDV) is a picornavirus that causes contagious acute infection in cloven-hoofed animals. FMDV replication-associated viral protein expression induces endoplasmic reticulum (ER) stress and the unfolded protein response (UPR), in turn inducing autophagy to restore cellular homeostasis. We
Tharun Selvam Mahendran et al.
Frontiers in aging neuroscience, 12, 537712-537712 (2020-12-01)
Aggregated tau is a hallmark neuropathological feature in numerous neurodegenerative disorders. Previous studies aiming to validate aggregated tau pathology as a pathogenic driver of neurodegeneration in correlation to characteristic behavioral phenotypes have had shortcomings. Although studies on soluble tau pathology
S N Suresh et al.
Frontiers in molecular neuroscience, 11, 109-109 (2018-04-25)
Mechanistic insights into aggrephagy, a selective basal autophagy process to clear misfolded protein aggregates, are lacking. Here, we report and describe the role of Estrogen Related Receptor α (ERRα, HUGO Gene Nomenclature ESRRA), new molecular player of aggrephagy, in keeping
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