K1637

Kanamycin sulfate from Streptomyces kanamyceticus

meets USP testing specifications, powder

• Synonim(y): Kanamycin A, Kanamycin sulfate salt
• Wzór empiryczny (zapis Hilla): C₁₈H₃₆N₄O₁₁ · H₂O₄S
• Numer CAS: 25389-94-0
• Masa cząsteczkowa: 582.58
• Numer WE: 246-933-9
• Numer MDL: MFCD00070253
• Kod UNSPSC: 51281654
• Identyfikator substancji w PubChem: 24896209
• NACRES: NA.76

Właściwości

• pochodzenie biologiczne: Streptomyces kanamyceticus
• Poziom jakości: 200
• agency: USP/NF; meets USP testing specifications
• Postać: powder
• siła działania: ≥750 μg per mg
• rozpuszczalność: H₂O: 10-50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 5 days.)
• spektrum działania antybiotyku: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• Zastosowanie: pharmaceutical (small molecule)
• Tryb działania: protein synthesis | interferes
• ciąg SMILES: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• Klucz InChI: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Opis

Opis ogólny

Kanamycin Sulfate is a broad spectrum aminoglycoside-antibiotic purified from the bacterium Streptomyces kanamyceticus. Kanamycin Sulfate is a selection agent for cells in culture media and acts by binding to the 30S subunit of the bacterial ribosome which inhibits protein synthesis in susceptible bacteria.

Zastosowanie

Kanamycin sulfate is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL. Kanamycin sulfate from Streptomyces kanamyceticus has been used: in the research of cell type-specific expression of genes

Działania biochem./fizjol.

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance:Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Cechy i korzyści

• High quality antibiotic suitable for mulitple research applications
• meets USP testing specifications

Uwaga dotycząca przygotowania

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Przechowywanie i stabilność

Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H360D
• Zwroty wskazujące środki ostrożności: P201 - P308 + P313
• Klasyfikacja zagrożeń: Repr. 1B
• Kod klasy składowania: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 2
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)