B3051

Brucella broth base

NutriSelect^® Plus, powder, selective for Brucella spp., selective for Campylobacter spp., pack of 500 g, suitable for microbiology

• NACRES: NA.85
• UNSPSC Code: 41171607
• Manufacturer/tradename: NutriSelect^® Plus
• Ph: 7.0±0.2 (25 °C)
• Form: powder
• Suitability: selective for Brucella spp., selective for Campylobacter spp.
• Application(s): clinical testing; environmental; food and beverages; sample preparation, microbiology

Właściwości

• sterility: non-sterile
• Quality Segment: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• pH: 7.0±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; sample preparation, microbiology
• suitability: selective for Brucella spp., selective for Campylobacter spp.

Opis

Application

Recommended for enrichment, isolation and cultivation of Brucella species.

Preparation Note

Suspend 14.05 g of Brucella Broth Base in 500 ml of distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add sterile 5% v/v heat inactivated (H1138) horse serum. Add polymyxin B (2,500 IU, Cat. No. 81334), bacitracin (12,500 IU, Cat. No. 11702), nystatin (50,000 IU, Cat. No. 74721), cycloheximide (50mg, Cat. No. 01810), nalidixic acid (2.5mg, Cat. No. 70162) and vancomycin (10mg, Cat. No. 94747) to make the medium selective. Mix well before pouring into sterile petri plates or tubes.
For isolation of the Campylobacter species add the rehydrated contents of one vial of Campylobacter Growth Supplement (Cat. No. C2847) and 5-7% defibrinated sheep blood to 500 ml of medium base. 500 ml of medium base. The Campylobacter Growth Supplement can also be made by mixing 125 mg sodium pyruvate (Cat. No. 15990), 125 mg sodium metabisulfite (Cat. No. 71928 )and 125 mg ferrous sulfate (Cat. No. 44970) in 2 ml of distilled water and filtering the solution through a sterile 0.4 mm filter to sterilize.

Other Notes

Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium bisulphite 0.10

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• ppe: Eyeshields, Gloves, type N95 (US)