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STEC Millichrome plus Supplement

suitable for microbiology

Synonim(y):

Shiga-Toxin producing E. coli (STEC) Chromogenic supplement

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Informacje o tej pozycji

NACRES:
NA.74
UNSPSC Code:
41171614
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sterility

aseptically filled

Quality Segment

form

dry powder

shelf life

limited shelf life, expiry date on the label

technique(s)

microbiological culture: suitable

solubility

sterile water: 1 (vial/10ml)

application(s)

food and beverages, microbiology

shipped in

ambient

storage temp.

2-8°C

suitability

selective for bacteria: Escherichia coli (Shiga Toxin-STEC

General description

STEC Millichrome plus Supplement is a selective media supplement for the detection of E. coli (STEC). The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli (STEC).Millichrome chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, each vial sufficient for 1 liter of final media. One vial of the supplement should be rehydrated with 10 mL of sterile water and then added to 1 liter of melted STEC Millichrome plus Agar base cooled at 45-50 °C.

Application

STEC Millichrome plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of E. coli (STEC) from food, animal feed, and environmental samples.

Packaging

5 vials (1 vial is sufficient for 1 L of final media)

Preparation Note

  • Aseptically rehydrate 1 vial with 10 mL of sterile water.
  • Swirl well until complete dissolution.
  • Add 1 vial rehydrated solution to 1L STEC Millichrome plus Agar base cooled at 45-50 °C.
  • Swirl gently to homogenize.

Analysis Note

The Shiga toxins E. coli is detected by a characteristic mauve color after only 24 h at an optimal growth temperature of 37°C, while most other Enterobacteriaceae form Colorless, blue, or inhibited after incubation at the same temperature

Legal Information

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
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Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Klasa składowania

11 - Combustible Solids

wgk

WGK 3



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