61767

Lectin from Triticum vulgaris

lyophilized, ≥55% (GE), powder

• Synonim(y): WGA, Wheat germ agglutinin
• UNSPSC Code: 12352202
• NACRES: NA.56
• MDL number: MFCD00147408
• Form: powder
• Biological source: Triticum vulgaris

Właściwości

• biological source: Triticum vulgaris
• form: powder
• quality: lyophilized
• concentration: ≥55% (GE)
• technique(s): agglutination assay: suitable using fresh human erythrocytes
• storage temp.: 2-8°C

Opis

General description

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.[1]

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and posses a carbohydrate binding sites on the surface.

Application

Wheat germ agglutinin conjugated to the outside surface of PLGA nanoparticles loaded with paclitaxel was used to target cancer cells that overexpress WGA-binding glycoproteins (Caco-2 and HT-29 cell lines). WGA-NP showed 50-60% greater endocytosis and intracellular retention of paclitaxel than unlabeled nanoparticles.[2]

Lectin from Triticum vulgaris has been used in bone resorption assays.

Biochem/physiol Actions

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.

Other Notes

Molecular characteristics and specificity for sugar binding[1]

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)