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WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Synonim(y):

HRP substrate, chemiluminescent detection substrate, detection substrate

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Gabaryty przesyłkiSKUDostępnośćCena netto
2 x 25 mL

Przewidywana wysyłka DZISIAJzKuehne + Nagel Sp. z o.o.

417,00 zł
2 x 50 mL

Przewidywana wysyłka DZISIAJzKuehne + Nagel Sp. z o.o.

716,00 zł
2 x 250 mL

Przewidywana wysyłka DZISIAJzKuehne + Nagel Sp. z o.o.

2090,00 zł

Informacje o tej pozycji

UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053

417,00 zł

Przewidywana wysyłka DZISIAJSzczegóły

​
Pomoc techniczna
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Pozwól nam pomóc

conjugate

peroxidase conjugate

Quality Segment

200

packaging

pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

5000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

General description

Immobilon Western HRP Substrate is compatible with all commonly used buffers and blocking reagents and provides high sensitivity for chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes.

Application

Immobilon Western Chemiluminescent HRP Substrate is suitable for western blotting.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WBULS0500WBLUCWBULS0100
Immobilon Western Chemiluminescent HRP Substrate Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Millipore

WBKLS

Immobilon Western Chemiluminescent HRP Substrate

Immobilon ECL Ultra Western HRP Substrate Western blotting, HRP

Millipore

WBULS0500

Immobilon ECL Ultra Western HRP Substrate

Immobilon Classico Western HRP substrate Western blotting, dot/slot blotting

Millipore

WBLUC

Immobilon Classico Western HRP substrate

Immobilon ECL Ultra Western HRP Substrate Western blotting, HRP

Millipore

WBULS0100

Immobilon ECL Ultra Western HRP Substrate

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

300

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

conjugate

peroxidase conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

-

manufacturer/tradename

Immobilon®

manufacturer/tradename

-

manufacturer/tradename

Luminata

manufacturer/tradename

-

Klasa składowania

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 2

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Protokoły

Liza komórek i ekstrakcja białek do Western Blotting

Protokół przygotowania próbki do lizy komórek i wydajnej ekstrakcji białek z hodowanych tkanek i komórek do późniejszego Western blottingu.

Usuwanie i odtwarzanie błon Western Blotting

Na tej stronie przedstawiono i omówiono trzy protokoły usuwania i reprobingu błony western blot.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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Produkty

Dot/Slot (Filtration) Blotting

The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Powiązane treści

Immobilon Western Chemiluminescent HRP Substrate
Luminata Crescendo ELISA HRP Substrate
History of Western Blotting

"Timeline of western blotting history. Timeless recipes and solutions for blotting success. Prepare and grow cell, media and sera with cultureware. Filter media with Stericup. ProteoExtract® kits for fractionation. CytoBuster™, BugBuster™ and CelLytic™ protein extraction reagents. Benzonase® nuclease. cOmplete™ and SIGMAFAST™ protease inhibitorsMaintain and preserve protein functionality following cell lysis using individual protease inhibitors and protease inhibitor cocktails. Amicon® filters for concentration and buffer exchange. PureProteome® magnetic beads for enrichment and purification. Recombinant protein purification affinity gels. Unlike methods that require careful aspiration to avoid sample loss, PureProteome® magnetic beads are isolated using a magnetic rack, for total removal of buffers and complete recovery with no sample dilution. TruPAGE™ precast gels Buffers (including Trizma®Tris base), solvents, prestained MW markers. Direct Detect® IR spectrometer for protein sample qualification. TruPAGE® precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. Range of Immobilon® PVDF transfer membranes. Western-validated antibodies. SNAP i.d.® 2.0 protein detection system. Immunodetection reagents. Blot efficiently using our Western blotting reagents, including Bløk® noise-cancelling reagents, Luminata™ Western HRP substrates, Immunoreaction Enhancer Reagents, and ReBlot™ Plus antibody stripping kits, all carefully optimized for your success."

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Numer pozycji handlu globalnego

SKUNUMER GTIN
WBKLS010008436037125102
WBKLS005008436037125140
WBKLS050004053252616754

Questions

  1. What is hydrogen peroxide concentration in Immobilon Western Chemiluminescent HRP Substrate, WBKLS0500?

    1 answer

    1. The concentration of the Hydrogen Peroxide component is considered proprietary, but has been optimized for use with the Luminol Reagent. Equal volumes of each component are required, as indicted in the table below.

      Please see the link below to review additional information available in the product user guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/865/668/p36599.pdf

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