SCC455

OSUMMER.11 Mouse NRAS-Mutant Melanoma Cell Line

• NACRES: NA.81
• UNSPSC Code: 41106514
• Growth mode: N/A
• Manufacturer/tradename: Millipore

Właściwości

• packaging: vial of ≥1X10⁶ cells/vial mg
• Quality Level: 100
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell analysis: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Opis

Application

Each vial contains 1X10⁶ viable cells.

Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel by Charles River Animal Diagnostic Services.

Cells are verified to be of mouse origin and negative for inter-species contamination from rat, human, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services

Cells are negative for mycoplasma contamination.

Early-stage melanoma can be cured by resection, but fewer treatment options are available for patients with metastatic disease. Development of resistance to targeted and immunotherapies, as well as toxicity are major problems. Along with sun exposure, sporadic genetic mutations have been associated with melanomas (1). Immunotherapy treatments targeting these genes or pathways have been proven to have significant benefits in melanoma patients by improving general response and survival.

The OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation) cell lines fill a previously unfilled gap in melanoma biology. The OSUMMER cell lines are syngeneic to C57BL/6 laboratory mice and have genetic profiles that are similar to human tumors, making them responsive to immunotherapy treatments. These NRAS-Mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-25% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model.

OSUMMER.11 mouse NRAS-mutant melanoma cell line was derived from a female TN mouse exposed to UVA and UVB radiation. OSUMMER.11 carries the specific NRAS mutation Q61R and can form tumors in vivo without Matrigel injection. WES revealed high SNV (Single Nucleotide variants) and low CNA (Copy Number Alterations) burden, as well as the presence of UV signature mutations. OSUMMER.11 cells express the melanoma markers PMEL and MelanA and exhibit robust growth in culture.

Source
OSUMMER.11 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (2).

References
1. Lancet 2018, 392(10151): 971-984.
2. Life Sci Alliance 2021, 4(9): e202101135.

Features and Benefits

The OSUMMER.11 cell line carries the specific NRAS mutation Q61R and may be used to create syngeneic mouse melanoma models.

Preparation Note

The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable