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MABT180

Sigma-Aldrich

Anti-MLC-2 Antibody, clone 19D3.1

clone 19D3.1, from mouse

Synonim(y):

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, MLC-2v

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified immunoglobulin

rodzaj przeciwciała

primary antibodies

klon

19D3.1, monoclonal

reaktywność gatunkowa

human, rat

metody

immunohistochemistry: suitable
western blot: suitable

izotyp

IgG1κ

numer dostępu NCBI

numer dostępu UniProt

Warunki transportu

wet ice

docelowa modyfikacja potranslacyjna

unmodified

informacje o genach

human ... MYL2(4633)

Opis ogólny

Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has several isoforms and is a component of myosin, which is made up of two heavy chains and four light chains. MLC2 is involved in smooth muscle contraction, stress fiber assembly, and cytokinesis. MLC2 defects can cause of familial hypertrophic cardiomyopathy type 10 (CMH10).

Specyficzność

Other homologies: Mouse 92% sequence homologies.

Immunogen

GST-tagged recombinant protein corresponding to human MLC-2.

Zastosowanie

Detect Myosin using this mouse monoclonal antibody, Anti-MLC-2 Antibody, clone 19D3.1 validated for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLC-2 in human cardiac myocyte and in rat skeletal muscle tissues.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Jakość

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected MLC-2 in 10 µg of human skeletal muscle tissue lysate.

Opis wartości docelowych

~19 kDa observed

Postać fizyczna

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Komentarz do analizy

Control
Human skeletal muscle tissue lysate.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Elena Chung et al.
Respiratory research, 21(1), 256-256 (2020-10-10)
Glucocorticoids (GCs) and β2-adrenergic receptor (β2AR) agonists improve asthma outcomes in most patients. GCs also modulate gene expression in human airway smooth muscle (HASM), thereby attenuating airway inflammation and airway hyperresponsiveness that define asthma. Our previous studies showed that the
Edwin J Yoo et al.
British journal of pharmacology, 174(23), 4383-4395 (2017-09-19)
PI3K-dependent activation of Rho kinase (ROCK) is necessary for agonist-induced human airway smooth muscle cell (HASMC) contraction, and inhibition of PI3K promotes bronchodilation of human small airways. The mechanisms driving agonist-mediated PI3K/ROCK axis activation, however, remain unclear. Given that G12
Christie A Ojiaku et al.
American journal of respiratory cell and molecular biology, 58(5), 575-584 (2017-10-07)
Transforming growth factor β1 (TGF-β1), a cytokine whose levels are elevated in the airways of patients with asthma, perpetuates airway inflammation and modulates airway structural cell remodeling. However, the role of TGF-β1 in excessive airway narrowing in asthma, or airway
Feng Liu et al.
Stem cell research & therapy, 11(1), 284-284 (2020-07-18)
Biological pacemakers derived from pluripotent stem cell (PSC) have been considered as a potential therapeutic surrogate for sick sinus syndrome. So it is essential to develop highly efficient strategies for enrichment of sinoatrial node-like cells (SANLCs) as seed cells for
Ernesto Cortes et al.
Hepatology (Baltimore, Md.), 69(2), 785-802 (2018-07-29)
Hepatic stellate cells (HSCs) are essential perisinusoidal cells in both healthy and diseased liver. HSCs modulate extracellular matrix (ECM) homeostasis when quiescent, but in liver fibrosis, HSCs become activated and promote excess deposition of ECM molecules and tissue stiffening via

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