MABS1183

Anti-Periostin Antibody, clone 5B2.3

clone 5B2.3, from mouse

• Synonim(y): Periostin, OSF-2, Osteoblast-specific factor 2, PN
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: 5B2.3, monoclonal
• reaktywność gatunkowa: human
• metody: immunohistochemistry: suitable (paraffin); western blot: suitable
• izotyp: IgG2aκ
• numer dostępu NCBI: NP_006466
• numer dostępu UniProt: Q15063
• Warunki transportu: ambient
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... POSTN(10631)

Opis

Opis ogólny

Periostin (UniProt Q15063; also known as OSF-2, Osteoblast-specific factor 2, PN) is encoded by the POSTN (also known as PSF2) gene (Gene ID 10631) in human. Periostin is an extracellular matrix (ECM) protein predominantly expressed in collagen-rich fibrous connective tissues, including heart valves, tendons, perichondrium, periosteum, and periodontal ligament (PDL). Periostin plays an essential role in the process of remodeling during tissue/organ development/repair and inflammation. Periostin derived from fibroblasts is shown to induce keratinocytes differentiation and proliferation. Periostin is a mediator for epidermal hyperplasia and is highly expressed in the dermis of both atopic dermatitis (AD) and psoriasis patients. Periostin level is also reported to be upregulated in patients with acute or chronic hepatitis and correlates well with serum TGF- 1 and TGF- 2 levels. The PDL-POSTN spliced isoform is predominantly expressed in PDL, it binds integrin v 3 with a higher affinity and induces strong activation of the integrin v 3-focal adhesion kinase (FAK) signaling pathway. Periostin is produced with a signal peptide (a.a. 1-21), the removal of which yields the mature protein with an N-terminal cysteine-rich EMI (a.a. 40-94) domain and four asciclin I or FAS1 domains (a.a. 97-230, 234-365, 368-492, 496-628). Alternative splicing generate several C-terminal domain variants with tissue-specific expression patterns.

Specyficzność

Clone 5B2.3 epitope lies within a region C-terminal to the FAS1 domains. Immunogen covered region is 100% present in isoforms 1 (OSF-2OS), 2 (OSF-2p1), and 5, but partially missing in spliced isoforms 3, 4 (PDL-POSTN), 6, and 7. Clone 5B2.3 immunoreactivity toward these latter isoforms is possible, but has not been confirmed.

Immunogen

GST-tagged recombinant human periostin C-terminal fragment.

Zastosowanie

Detect Periostin using this mouse monoclonal Anti-Periostin, clone 5B2.3, Cat. No. MABS1183, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected periostin in human prostate cancer, breast and small intestine tissue sections.

Research Category
Signaling

Jakość

Evaluated by Western Blotting in human stomach tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected periostin in 10 µg of human stomach tissue lysate.

Opis wartości docelowych

~78-93 kDa observed. The broad band appearance is consistent with the detetion of differentially glycosylated species of one or more spliced isoforms. 90.96/87.021/84.67/81.50/88.07/81.17/78.00 kDa (mature isoform 1/2/3/4/5/6/7) and 93.31/87.02/87.25/83.85/90.42/83.53/80.36 kDa (isoform 1/2/3/4/5/6/7 pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).

Postać fizyczna

Format: Purified

Protein G purified.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Przechowywanie i stabilność

Stable for 1 year at 2-8°C from date of receipt.

Inne uwagi

Concentration: Please refer to lot specific datasheet.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable