MABE917

Anti-DNA G-quadruplex structures Antibody, clone BG4

clone BG4, from Escherichia coli

• Synonim(y): DNA G-quadruplex (BG4)
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: BG4, monoclonal
• Species reactivity: human
• Application: ChIP, ELISA, ICC
• Citations: 23

Właściwości

• biological source: Escherichia coli
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: BG4, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μL
• technique(s): ChIP: suitable (ChIP-seq), ELISA: suitable, immunocytochemistry: suitable
• shipped in: ambient
• target post-translational modification: unmodified

Opis

General description

DNA G-quadruplex structures are associated with a number of important aspects of genome function, which include transcription, recombination and replication. Single-stranded guanine (G)-rich DNA can form stable secondary structures called G-quadruplexes (G-tetrads or G4), which can influence methylation at CpG islands (CGIs). G4 structure is reported to be tightly associated with CGI hypomethylation in the human genome. Four guanine bases can associate through Hoogsteen hydrogen bonding to form a square planar structure called a guanine tetrad (G-tetrad), and two or more G-tetrads can stack on top of each other to form a G-quadruplex. The quadruplex structure is further stabilized by the presence of a cation, especially potassium, which sits in a central channel between each pair of tetrads. A DNA G-tetrad can be formed within one DNA strand (intramolecular), between two DNA strands (bimolecular), or four DNA strands (tetramolecular). G4 DNA can arise anywhere in the genome where sufficiently long stretches of single-stranded G-rich DNA are exposed during replication, transcription or recombination. Chemical analysis of quadruplex-forming oligonucleotides has revealed the existence of a plethora of dynamic quadruplex structures with varying stabilities. The diverse nature of G4 DNA structures makes it an attractive topic in molecular biomedical research.(Ref.: Mao, SQ et al., (2018). Nat. Struc. Mol. Biol. 25(10); 951-957).

Immunogen

G-quadruplex oligonucleotides.

Application

Anti-DNA G-quadruplex structures, clone BG4 Antibody, Cat. No. MABE917, is a highly specific monoclonal antibody that targets DNA G-quadruplex structures and has been tested in and ELISA, ChIP-seq, and Immunocytochemistry.

ELISA Analysis: A representative lot detected DNA G-quadruplex structures in Anti-DNA G-quarduplex structures, BG4 (Courtesy of University of Cambridge).

ChIP-seq Analysis: A representative lot detected DNA G-quadruplex structures in ChIP-seq applications (Hansel-Hertsch, R., et. al. (2016). Nat Genet. 48(10):1267-72).

ELISA Analysis: A representative lot detected DNA G-quadruplex structures in ELISA applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).

Immunocytochemistry Analysis: A representative lot detected DNA G-quadruplex structures in Immunocytochemistry applications (Biffi, G., et. al. (2013). Nat Chem. 5(3):182-6).

Biochem/physiol Actions

Clone BG4 has a high affinity for intramolecular and intermolecular DNA G-quadruplexes with no detectable binding to a RNA hairpin, single-stranded DNA or double-stranded DNA.

Physical form

Format: Purified

Purified monoclonal antibody in PBS with 0.05% sodium azide.

Analysis Note

The Anti-DNA-G-quadruplex structures, clone BG4 antibody (Cat. No. MABE917) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with Anti-FLAG epitope tag antibody (MAB3118).

Other Notes

Concentration: Please refer to lot specific datasheet.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable