MAB8131

Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2

clone 6F8.2, Chemicon^®, from mouse

• Synonim(y): CMV
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 6F8.2, monoclonal
• Species reactivity: human
• Application: ICC, IF, IHC, WB
• Citations: 15

Właściwości

• biological source: mouse
• Quality Segment: 300
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6F8.2, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG2a
• shipped in: wet ice

Opis

Immunogen

Affinity purified early antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Epitope: immediate early

Application

Anti-Cytomegalovirus Antibody, immediate early, clone 6F8.2 is an antibody against Cytomegalovirus for use in IF, WB, IC, IH(P).

Immunohistochemistry.

Immunocytochemistry.

Western Blot 1:100

IFA at 1:600-1:1,200 on acetone fixed cells.

Works on paraffin embedded tissue sections.

Final working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Biochem/physiol Actions

Reacts with an immediate early protein M.W. 68-72 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and/or intranuclear inclusion staining which reaches peak intensity between 8-24 hours.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

Physical form

Format: Purified

Purified immunoglobulin. Supplied in PBS buffer, pH 7.4. Contains 0.1% sodium azide and carrier protein. Has been sterile filtered.

Preparation Note

Maintain at +4°C for up to 12 months

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB8130

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Klasa składowania: 12 - Non Combustible Liquids