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MAB5310

Anti-Glutathione Antibody, clone D8

clone D8, Chemicon®, from mouse

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Gabaryty przesyłkiSKUDostępnośćCena netto
100 μg

Przewidywana wysyłka DZISIAJzKuehne + Nagel Sp. z o.o.

2450,00 zł

Informacje o tej pozycji

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
D8, monoclonal
Species reactivity:
eukaryotes
Application:
WB
Citations:
14

2450,00 zł


Przewidywana wysyłka DZISIAJSzczegóły


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biological source

mouse

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

D8, monoclonal

species reactivity

eukaryotes

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

IgG2a

shipped in

dry ice

target post-translational modification

unmodified

Immunogen

Glutathione-protein complexes.

Application

Anti-Glutathione Antibody, clone D8 is an antibody against Glutathione for use in WB.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress
Western blotting of glutatione conjugated proteins under non-reduced conditions: 1:500-1:1000; does not react with glutatione by itself.

Immunocytochemistry: 1:500-1:1000 (Non-reducing conditions)

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Reacts with reduced or non-reduced glutathione on proteins only. Does not react with free glutathione.

Physical form

Format: Purified
Immunoglobulin purified by protein A affinity and presented as a liquid in PBS, pH 7.2 with 0.01% sodium azide.

Preparation Note

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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biological source

mouse

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mouse

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rabbit

antibody form

affinity purified immunoglobulin

antibody form

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antibody form

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antibody form

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clone

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clone

polyclonal

technique(s)

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technique(s)

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technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

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isotype

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isotype

IgG2a

isotype

IgG2a

isotype

-


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Klasa składowania

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



Certyfikaty analizy (CoA)

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Powiązane treści

"Redox reactions are powerful chemical processes that involve the reduction and oxidation of proteins and metabolites found in living things. The mechanisms that regulate them are key to maintaining homeostasis and the balance between good health and disease pathology. Oxidative stress is the state where the delicate balance of redox biology is upset, and the pathology of oxidative stress are the cellular consequences to such an imbalance."

"Aging: getting older, exhibiting the signs of age, the decline in the physical (and mental) well-being over time, leading to death. Since the beginning of time, man has been obsessed with trying to slow down, stop, or even reverse the signs of aging. Many have gone as far as experimenting with nutritional regimens, eccentric exercises, fantastic rituals, and naturally occurring or synthetic wonder-elements to evade the signs of normal aging. Biologically speaking, what is aging? And what does the latest research tell us about the possibility of discovering the elusive “fountain of youth”? Many advances in our understanding of aging have come from systematic scientific research, and perhaps it holds the key to immortality. Scientifically, aging can be defined as a systems-wide decline in organismal function that occurs over time. This decline occurs as a result of numerous events in the organism, and these events can be classified into nine “hallmarks” of aging, as proposed by López-Otin et al. (2013). Several of the pathologies associated with aging are a direct result of these events going to extremes and may also involve aberrant activation of proliferation signals or hyperactivity. The hallmarks of aging have been defined based on their fulfillment of specific aging related criteria, such as manifestation during normal aging, acceleration of aging if experimentally induced or aggravated, and retardation of aging if prevented or blocked, resulting in increased lifespan. The nine hallmarks of aging are genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, and altered intercellular communication. The biological processes underlying aging are complex. By understanding the hallmarks in greater detail, we can get closer to developing intervention strategies that can make the aging process less of a decline, and more of a recline."


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Numer pozycji handlu globalnego

SKUNUMER GTIN
MAB531004053252610394

Questions

  1. Hi, we would like to see if this antibody works for drosophila samples. Is it possible to order a small samples?  In addition, does adding SDS in the protein preparation interrupt the use of this antibody? Thanks, Liping

    1 answer
    1. The species reactivity of this antibody is Eukaryote; it is expected to react with Drosophila. The Western blotting analysis of glutathione conjugated proteins was performed under non-reduced conditions. Interference in the presence of sodium dodecyl sulfate has not been investigated. Note that this antibody does not react with free glutathione.

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