MAB5272

Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324

clone 324, Chemicon^®, from rat

• Synonim(y): CD171, N-CAM L1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 324, monoclonal
• Application: ICC, IHC, WB
• Citations: 74

Właściwości

• biological source: rat
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 324, monoclonal
• species reactivity: mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_032504.3
• UniProt accession no.: P11627
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... L1CAM(3897), VCAM1(7412)

Opis

General description

120-140 kDa

Families of adhesion molecules which share common carbohydrate domains do exist, despite the structural and functional diversity of these glycoproteins. These include the Ca2+-independent neural adhesion molecules: N-CAM, myelin associated glycoprotein (MAG) and L1. L1 is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts

Immunogen

Rats were immunized with a glycoprotein fraction from cerebellum of 8-10 day old C57BL/6J mice.

Application

This Anti-Neural Cell Adhesion Molecule L1 Antibody, clone 324 is validated for use in IC, IH, WB for the detection of Neural Cell Adhesion Molecule L1.

Western blotting:
20 μg/mL was used on a previous lot.

Immunocytochemistry:
5-10 μg/mL was used on a previous lot. Cells must be fixed with methanol for 10 minutes at -20°C in the middle log phase.

Immunohistochemistry: Clone 324 is sensitive to fixation. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Monoclonal antibody against neural cell adhesion molecule L1 from rat-rat hybrid cells.

Physical form

Format: Purified

Rat monoclonal in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Klasa składowania: 10 - Combustible liquids
• wgk: WGK 2