Yes, it is possible to use two primary antibodies from different species but of the same isotype for double staining Immunofluorescence. Specifically, for MAB397 and GluR2, one can use an anti-mouse and an anti-rabbit secondary antibody to distinguish reactivity of the primary antibodies.
MAB397
Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4
clone 6C4, Chemicon®, from mouse
Synonim(y):
GluR2
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| Gabaryty przesyłki | SKU | Dostępność | Cena netto |
|---|---|---|---|
| 100 μg | Dostępny do wysyłki w dniu06 maja 2026zKuehne + Nagel Sp. z o.o. | 2710,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
6C4, monoclonal
Application:
ELISA, ICC, IHC, IP, RIA, WB
Citations:
404
2710,00 zł
Dostępny do wysyłki w dniu06 maja 2026Szczegóły
Rekombinowane, niezawierające konserwantów przeciwciało jest dostępne dla Twojego celu. Wypróbuj ZRB1008
Pomoc techniczna
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Pozwól nam pomócbiological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6C4, monoclonal
species reactivity
monkey
species reactivity (predicted by homology)
mouse, rat, canine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
dog ... Gria2(482667)
mouse ... Gria2(14800)
rat ... Gria2(29627)
rhesus monkey ... Gria2(574344)
General description
Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
102 kDa
Application
Anti-Glutamate Receptor 2 Antibody, extracellular, clone 6C4 detects level of Glutamate Receptor 2 & has been published & validated for use in ELISA, IC, IH, IP, RIA & WB with more than 50 product citations.
Immunocytochemistry:
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).
ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).
Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).
Immunoprecipitation: 2-4 µg/mL (Osten, 1998).
Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.
Optimal working dilutions and protocols must be determined by end user.
on 4% paraformaldehyde fixed cells was used in a previous lot:2-3 µg/mL (Vissavajjhala, 1996; Osten, 1998; Passafaro, 2001).
ELISA/RIA:
A previous lot of this antibody was used in ELISA/RIA.(Vissavajjhala, 1996).
Immunohistochemistry on 50 µm, 4% paraformaldehyde fixed brain sections: 1:500-1:800 (Vissavajjhala, 1996; Yung, 1998; Kumar; 2002).
Immunoprecipitation: 2-4 µg/mL (Osten, 1998).
Western blot: 1-2 µg/mL (Vissavajjhala, 1996; Osten, 1998); membrane preparations are suggested for enhanced signals.
Optimal working dilutions and protocols must be determined by end user.
Biochem/physiol Actions
Recognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits. On western blots of brain extracts from rat, macaque monkey, and dog, MAB397 recognizes a band at approximately 102 kDa corresponding to full length GluR2. Other proteins noted by Western blot at 66 kDa or lower molecular weight appear to be breakdown products of GluR2 (Vissavajjhala, 1996). By immunohistochemistry GluR2 is widely distributed at both the cellular and synaptic levels. MAB397 recognizes GluR2 present in a vast majority of, but not all, GABAergic interneurons (Vissavajjhala, 1996).
Physical form
Format: Purified
Purified immunoglobulin from culture supernatant
Purified mouse immunoglobin IgG2a liquid in buffer containing PBS, no preservative.
Preparation Note
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage the IgG2a and affect product performance.
Analysis Note
Control
Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
Positive Control: Cerebral cortex and pyramidal neurons, mouse brain lysate.
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected glutamate receptor 2 on 10 μg of mouse brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form serum | antibody form purified antibody | antibody form purified immunoglobulin |
| species reactivity monkey | species reactivity mouse, rat | species reactivity rat | species reactivity rat |
| biological source mouse | biological source rabbit | biological source mouse | biological source mouse |
| clone 6C4, monoclonal | clone polyclonal | clone L21/32, monoclonal | clone RH95, monoclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| Gene Information dog ... Gria2(482667) | Gene Information human ... GRIA2(2891) | Gene Information human ... GRIA2(2891) | Gene Information human ... GRIA1(2890) |
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Klasa składowania
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Powiązane treści
Glutamate is an excitatory neurotransmitter found in the synaptic vesicles of glutamatergic synapses. The post-synaptic neurons in these synapses contain ionotropic and metabotropic glutamate receptors. Glutamate binds to AMPA (α-amino-3-hydroxy-5- methylisoxazole-4-propionic acid) subtype glutamate receptors, leading to sodium influx into the post-synaptic cell and resulting in neuronal excitability and synaptic transmission. The NMDA (N-methyl-d-aspartate) subtype glutamate receptors, on the other hand, regulate synaptic plasticity, and can influence learning and memory. The metabotropic g-protein coupled mGluRs modulate downstream calcium signaling pathways and indirectly influence the synapse’s excitability. The synaptic architecture includes intracellular scaffolding proteins (PSD-95, GRIP), intercellular cell adhesion molecules (NCAMs, N-Cadherins), and a variety of signaling proteins (CaMKII/PKA, PP1/PP2B). Processes critical for synaptic transmission and plasticity are influenced by these molecules and their interactions. When the function of these molecules is disrupted, it leads to synaptic dysfunction and degeneration, and can contribute to dementia as seen in Alzheimer’s disease.
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors.
Danielson, E; Zhang, N; Metallo, J; Kaleka, K; Shin, SM; Gerges, N; Lee, SH
The Journal of Neuroscience null
Differential regulation of AMPA receptor and GABA receptor trafficking by tumor necrosis factor-alpha.
Stellwagen, D; Beattie, EC; Seo, JY; Malenka, RC
The Journal of Neuroscience null
Tyrosine phosphatases regulate AMPA receptor trafficking during metabotropic glutamate receptor-mediated long-term depression.
Moult, PR; Gladding, CM; Sanderson, TM; Fitzjohn, SM; Bashir, ZI; Molnar, E; Collingridge, GL
The Journal of Neuroscience null
Numer pozycji handlu globalnego
| SKU | NUMER GTIN |
|---|---|
| MAB397 | 04053252463792 |
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Is it possible to use two primary antibodies from different species but of the same isotype for double staining Immunofluorescence? Specifically, considering MAB397 and GluR2.
1 answer-
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